Thioredoxin-like 5/TRP14/TXNDC17 Antibody [Unconjugated]

Western blot shows lysates of A20 mouse B cell lymphoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse/Rat Thioredoxin-like 5/TRP14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3769) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Thioredoxin-like 5/TRP14 at approximately 14 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.

• Reactivity: Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Thioredoxin-like 5/TRP14/TXNDC17 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse Thioredoxin-like 5/TRP14
  Met1-Asp123
  Accession # Q9CQM5
• Specificity: Detects endogenous mouse and rat Thioredoxin-like 5/TRP14 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TXNDC17
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Thioredoxin-like 5/TRP14/TXNDC17 Antibody [Unconjugated]

• MGC14353
• Protein 42-9-9
• thioredoxin (Trx)-related protein, 14 kDa
• thioredoxin domain containing 17
• thioredoxin domain-containing protein 17
• Thioredoxin like 5
• Thioredoxin-like 5
• Thioredoxin-like protein 5
• TRP14
• TRP1414 kDa thioredoxin-related protein
• TXNDC17
• TXNL5

Background

Thioredoxin-like 5, also known as a 14 kDa thioredoxin-related protein (TRP14) is a 14 kDa cytosolic redox regulatory molecule that belongs to the thioredoxin family. It contains a thioredoxin-like redox-sensitive active site motif (Cys-Pro-Asp-Cys). In its reduced form, the active site cysteine residues reduce protein disulfide bonds. The resulting active site disulfide is subsequently reduced in a reaction catalyzed by the NADPH-dependent thioredoxin reductase 1. Thioredoxin-like 5 is ubiquitously expressed and acts on protein targets different from that of Thioredoxin-1. The amino acid sequence of mouse Thioredoxin-like 5 is 96% identical to that of rat Thioredoxin-like 5.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TXNDC17
• Uniprot:
  • Q9CQM5()