Thioredoxin-2 Antibody (543317)

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, CH-1 mouse B cell lymphoma cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat Thioredoxin-2 Monoclonal Antibody (Catalog # MAB5765) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Thioredoxin-2 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line and L6 rat myoblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Thioredoxin‑2 at approximately 11 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human/Mouse/Rat Thioredoxin‑2 Monoclonal Antibody (Catalog # MAB5765). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western
• Clone: 543317
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Thioredoxin-2 Antibody (543317) Summary

• Immunogen: E. coli-derived recombinant human Thioredoxin-2
  Thr60-Gly166
  Accession # Q99757
• Specificity: Detects human, mouse and rat Thioredoxin-2 in Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TXN2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Simple Western 5 ug/mL
  • Western Blot 0.5 ug/mL
• Application Notes: In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Thioredoxin-2 Antibody (543317)

• mitochondrial thioredoxin
• MTRX
• MT-TRX
• thioredoxin 2
• thioredoxin, mitochondrial
• Thioredoxin2
• Thioredoxin-2
• Trx2
• TXN2

Background

Thioredoxin-2 (Trx-2; also known as mitochondrial Thioredoxin) belongs to the evolutionarily conserved Thioredoxin family of proteins. Thioredoxins share the Thioredoxin fold containing the active site-CGPC motif. In their reduced form, the active site cysteine residues reduce protein disulfides. The resulting active site disulfide is subsequently reduced in a reaction catalyzed by a NADPH-dependent Thioredoxin reductase. Thioredoxin-2 contains an N-terminal 59 amino acid (aa) transit sequence that is cleaved upon translocation to mitochondria. The amino acid sequence of mature human Thioredoxin-2 is 98% identical to mouse and rat Thioredoxin-2. Thioredoxin-2 interacts with specific components of the mitochondrial respiratory chain and helps regulate the membrane potential. Thioredoxin-2 is ubiquitously but variably expressed and high expression confers resistance to oxidant-induced apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Thioredoxin-2 Antibody (MAB5765)(1)

Publication using MAB5765

• MJ Kim, C Han, K White, HJ Park, D Ding, K Boyd, C Rothenberg, U Bose, P Carmichael, PJ Linser, M Tanokura, R Salvi, S Someya Txn2 haplodeficiency does not affect cochlear antioxidant defenses or accelerate the progression of cochlear cell loss or hearing loss across the lifespan Exp. Gerontol., 2020-08-28;141(0):111078. 2020-08-28 [PMID: 32866605] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: TXN2
• Uniprot:
  • Q99757(Human)