TGR5/GPBAR1 Antibody (409522) [Allophycocyanin]

HEK293 human embryonic kidney cell line transfected with human TGR5/GPBAR1 and eGFP was stained with either (A) Mouse Anti-Human TGR5/GPBAR1 APC-conjugated Monoclonal Antibody (Catalog # FAB4286A) or (B) Mouse IgG2BAllophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 409522
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

TGR5/GPBAR1 Antibody (409522) [Allophycocyanin] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human TGR5/GPBAR1
  Met1-Asn330
  Accession # Q8TDU6
• Specificity: Stains human TGR5/GPBAR1 transfectants but not irrelevant transfectants in flow cytometry.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: GPBAR1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TGR5/GPBAR1 Antibody (409522) [Allophycocyanin]

• BG37
• BG37hBG37
• G protein-coupled bile acid receptor 1
• GPBA
• GPBAR1
• GPCR19
• GPR131
• G-protein coupled bile acid receptor 1
• G-protein coupled bile acid receptor BG37
• G-protein coupled receptor GPCR19
• M-BAR
• M-BARhGPCR19
• membrane bile acid receptor
• Membrane-type receptor for bile acids
• MGC40597
• TGR5
• TGR5GPCR

Background

TGR5, also known as GPR131, GPBAR1, M-BAR, and BG37, is a 46-48 kDa member of the GPCR1 family of molecules. It is a 330 amino acid (aa) 7-transmembrane glycoprotein that is found on a wide variety of cell types. These include monocytes, macrophages, Kupffer cells, skeletal muscle and pancreatic acinar cells, autonomic enteric and sensory DRG neurons, liver sinusoidal endothelial cells, intestinal L and EC (enterochromaffin) cells, gallbladder epithelium and cholangiocytes (bile duct epithelium). Notably, TGR5 expression exhibits polarity with respect to plasma membrane location, and it may appear either on the cell surface or in intracellualr membrane compartments such as the ER, nucleus or multivesicular bodies. Initially considered to be coupled to G alpha s with cAMP production, it is now known to also interact with G alpha i, activating the ERK signaling pathway. Bile acids generated from cholesterol in hepatocytes are a known ligand for TGR5. Aside from their role in fatty acid absorption, BA ligation of macrophage/Kupffer cell TGR5 results in an anti-inflammatory environment. Over the aa that constitute the TGR5 extracellular domain, human and mouse share 73% aa sequence identity; over the entire ORF, human and mouse share 83% aa sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GPBAR1
• Uniprot:
  • Q8TDU6()