TfR (Transferrin R) Antibody (23D10) - BSA Free

ELISA: Transferrin Receptor Antibody (23D10) [NB110-8468] - Titration curve of purified soluble transferrin receptor (sTfR) using pair 23D10-13E4. Capture: MAb 23D10; 200 ng/well Detection: MAb 13E4 conjugated with HRP Room temperature

ELISA: Transferrin Receptor Antibody (23D10) [NB110-8468] - Titration curve of sTfR-Tf complex contained in normal human serum using sTfR-Tf assay. Capture: MAb 23D10; 200 ng/well Detection: MAb 8B9 or 11D3 conjugated with HRP Room temperature

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 23D10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

TfR (Transferrin R) Antibody (23D10) - BSA Free Summary

• Immunogen: Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with purified human soluble transferrin receptor.
• Localization: Cell Membrane and Secreted
• Marker: Recycling Endosome Marker
• Specificity: Human soluble transferrin receptor and complex between soluble transferrin receptor and transferrin.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: TFRC
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Sandwich ELISA 1:100-1:2000
  • Western Blot 1:100-1:2000
• Application Notes: Recommended pairs for ELISA (capture - detection): NB110-8468 - NB100-73092

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Purity: Protein G purified

Alternate Names for TfR (Transferrin R) Antibody (23D10) - BSA Free

• CD71 antigen
• CD71
• CD71TRFR
• p90
• T9
• TfR (Transferrin R)
• TfR (TransferrinR)
• TfR
• TFR1
• TFRC
• TR
• transferrin receptor (p90, CD71)
• transferrin receptor protein 1
• TRFR

Background

Cellular uptake of iron occurs via receptor mediated endocytosis of ligand occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). Useful in studies of dividing haematopoietic and tumour cell populations, and metabolic activity. A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C terminal binding site. The antigen is present on most dividing cells, including normally cycling in vivo hematopoietic progenitor cells, mitogenically stimulated cells in vitro, some primary tumor cells and most proliferating cells in vitro. Transferrin Receptor Antibody (23D10) has been structurally characterized as a sulfide bound dimer of identical glycoprotein subunits of 95 kDa. Transferrin Receptor Antibody (23D10) is not present on resting blood lymphocytes. On PBL, the receptor appears after activation. The expression of transferrin receptor is coordinately regulated with cell growth. Present on T and B cell lines. The soluble (or serum) transferrin receptor (sTfR) is a circulating truncated form of the membrane receptor protein; it is an 85 kDa glycoprotein forming in serum a 320 kDa complex with diferric transferrin.The most important clinical use of the sTfR determination is in the differential diagnosis between iron deficiency anaemia and the anaemia of chronic disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TFRC
• Entrez:
  • 7037(Human)