TfR (Transferrin R) Antibody (13E4cc) - BSA Free

ELISA: TfR (Transferrin R) Antibody (13E4) [NB100-73092] - Titration curve of purified soluble transferrin receptor (sTfR) using pair 23D10 - 13E4. Capture: MAb 23D10 (NB110-8468); 200 ng/well Detection: MAb 13E4 conjugated with HRP Room temperature

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 13E4cc
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

TfR (Transferrin R) Antibody (13E4cc) - BSA Free Summary

• Immunogen: Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with purified human soluble transferrin receptor.
• Marker: Recycling Endosome Marker
• Specificity: This is specific to human soluble transferrin receptor and complex between soluble tansferrin receptor and transferin.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: TFRC
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Sandwich ELISA 1:100-1:2000
  • Western Blot 1:100-1:2000
• Application Notes: Best pair (capture-detection): NB110-8468 - NB100-73092

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Purity: Protein A purified

Alternate Names for TfR (Transferrin R) Antibody (13E4cc) - BSA Free

• CD71 antigen
• CD71
• CD71TRFR
• p90
• T9
• TfR (Transferrin R)
• TfR (TransferrinR)
• TfR
• TFR1
• TFRC
• TR
• transferrin receptor (p90, CD71)
• transferrin receptor protein 1
• TRFR

Background

Cellular uptake of iron occurs via receptor mediated endocytosis of ligand occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). Useful in studies of dividing haematopoietic and tumor cell populations, and metabolic activity. A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C terminal binding site. The antigen is present on most dividing cells, including normally cycling in vivo hematopoietic progenitor cells, mitogenically stimulated cells in vitro, some primary tumor cells and most proliferating cells in vitro. The transferrin receptor has been structurally characterized as a sulfide bound dimer of identical glycoprotein subunits of 95 kDa. The transferrin receptor is not present on resting blood lymphocytes. On PBL, the receptor appears after activation. The expression of transferrin receptor is coordinately regulated with cell growth. Present on T and B cell lines. The soluble (or serum) transferrin receptor (sTfR) is a circulating truncated form of the membrane receptor protein; it is an 85 kDa glycoprotein forming in serum a 320 kDa complex with diferric transferrin. The most important clinical use of the sTfR determination is in the differential diagnosis between iron deficiency anemia and the anemia of chronic disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TfR (Transferrin R) Antibody (NB100-73092)(4)

Publications using NB100-73092

• Cui F, Mi H, Wang R et al. The effect of chronic intermittent hypobaric hypoxia improving liver damage in metabolic syndrome rats through ferritinophagy Research Square 2023-05-24 (WB, Rat)
• Yin J, Niu J, Huo J et al. Construction and Evaluation of a Novel MAP Immunoassay for 9 Nutrition-and-Health-Related Protein Markers Based on Multiplex Liquid Protein Chip Technique Nutrients 2023-03-21 [PMID: 36986252]
• Halbert D, Domenyuk V, Spetzler D et al. Aptamers and uses thereof United States Patent Application US 9958448 B2 2018-01-01
• Kitamura K, Okamoto A, Morio H et al. Human Immortalized Cell-Based Blood-Brain Barrier Spheroid Models Offer an Evaluation Tool for the Brain Penetration Properties of Macromolecules Molecular pharmaceutics 2022-08-01 [PMID: 35766901]

Bioinformatics

• Gene Symbol: TFRC
• Entrez:
  • 7037(Human)