TFEB Antibody

Product Discontinued

TFEB Antibody Summary

• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human TFEB. The exact sequence is proprietary.
• Localization: Nucleus
• Predicted Species: Rat (100%), Bovine (100%), Rabbit (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TFEB
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:3000
• Theoretical MW: 53 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 30706760).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for TFEB Antibody

• ALPHATFEB
• BHLHE35
• Class E basic helix-loop-helix protein 35
• T-cell transcription factor EB
• TCFEB
• TCFEBbHLHe35
• TFEB
• transcription factor EB

Background

Transcription factors of the basic helix-loop-helix zipper (bHLH-ZIP) family contain a basic domain, used for DNA binding, and HLH and ZIP domains, both used for oligomerization. TFEB was isolated from a human B-cell cDNA library using a binding sequence from the adenovirus major late promoter. This transcription factor probably binds to the USF/MLTF site and probably recognizes E-box sequences in the heavy-chain immunoglobulin enhancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for TFEB Antibody (NBP1-30908)(1)

Publication using NBP1-30908

• Ye B, Wang Q, et al. Restoring autophagic flux attenuates cochlear spiral ganglion neuron degeneration by promoting TFEB nuclear translocation via inhibiting MTOR. Autophagy 2019-06-01 [PMID: 30706760] (WB, Mouse)

Bioinformatics

• Gene Symbol: TFEB