Home » TFB1M » TFB1M Antibodies » TFB1M Antibody

TFB1M Antibody

 (1 Publication)
 See All Related
Submit a Review

Images

 
(
Enlarge)
Western Blot: TFB1M Antibody [NBP1-51978] - (0.3ug/ml) staining of A431 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: TFB1M Antibody [NBP1-51978] - (0.3ug/ml) staining of A431 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

100% Guarantee on every product

TFB1M Antibody Summary

Immunogen
Peptide with sequence C-ELKRRKSKNEEKE corresponding to C-Terminus according to NP_057104.2.
Epitope
C-ELKRRKSKNEEKE
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TFB1M
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 0.3 - 1 ug/ml
  • Peptide ELISA Detection limit 1:64000
Application Notes
WB: Approx. 38 kDa band observed in A431 lysates (calculated MW of 39.5 kDa band according to NP_057104.2).
Publications
Read Publication using NBP1-51978.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Canine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for TFB1M Antibody

  • CGI75
  • CGI-75
  • dimethyladenosine transferase 1, mitochondrial
  • EC 2.1.1
  • EC 2.1.1.-
  • h-mtTFB
  • h-mtTFB1
  • homolog of yeast mitochondrial transcription factor B
  • hTFB1M
  • Mitochondrial 12S rRNA dimethylase 1
  • mitochondrial dimethyladenosine transferase 1
  • Mitochondrial transcription factor B1
  • mtTFB
  • mtTFB1
  • S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase 1
  • transcription factor B1, mitochondrial

Background

The transcription of genes from mitochondrial DNA requires a mitochondrial RNA polymerase (see POLRMT, MIM 601778) and a DNA-binding transcription factor (see TFAM, MIM 600438). Transcription factor B1 (TFB1M) is a part of this transcription complex.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

TFB2M Antibody
NBP1-82114
Western Blot: TFB2M Antibody [NBP1-82114] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry: TFB2M Antibody [NBP1-82114] - Staining of human heart muscle shows moderate cytoplasmic positivity in myocytes.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
mtTFA Antibody
(18G102B2E11)
NBP1-71648
Western Blot: mtTFA Antibody (18G102B2E11) [NBP1-71648] - Alcohol feeding significantly decreased TFAM expression in rat liver. This image was submitted by a customer review.
Immunocytochemistry/Immunofluorescence: mtTFA Antibody (18G102B2E11) [NBP1-71648] - Immunostaining in HeLa cells. C1: DAPI staining 2 nuclei, C2: anti-mtTFA stain with Alexa Fluor 488 secondary ab (green), C3: Mitotracker Red staining mitochondria. Image courtesy of Elizabeth Wang.
Immunohistochemistry-Paraffin: mtTFA Antibody (18G102B2E11) [NBP1-71648] - IHC staining of mtTFA on human testis using DAB with hematoxylin counterstain.
(1 Review)
(6 Publications)
Species: Hu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
SPANXB1 Antibody
H00728695-B01P
Western Blot: SPANXB1 Antibody [H00728695-B01P] - Analysis of SPANXB1 expression in transfected 293T cell line by SPANXB1 polyclonal antibody. Lane 1: SPANXB1 transfected lysate(11.33 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/Immunofluorescence: SPANXB1 Antibody [H00728695-B01P] - Analysis of purified antibody to SPANXB1 on Hs 181.Tes cell. (antibody concentration 10 ug/ml)
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC-P
BBS9 Antibody
NBP1-88927
Immunocytochemistry/Immunofluorescence: BBS9 Antibody [NBP1-88927] - Staining of human cell line A-431 shows positivity in nucleus but not nucleoli, plasma membrane and cytoplasm.
Immunohistochemistry-Paraffin: BBS9 Antibody [NBP1-88927] - Staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.
(3 Publications)
Species: Hu
Applications: ICC/IF, IHC, IHC-P
CD20 Antibody
(AISB12)
NBP1-43435
Western Blot: MS4A1/CD20 Antibody (AISB12) [NBP1-43435] - Immunoblot of Balb/c thymus (lane 1) and A20 (lane 2) cell lysates with Anti-Mouse CD20 Purified.
Flow Cytometry: MS4A1/CD20 Antibody (AISB12) [NBP1-43435] - Staining of BALB/c splenocytes with Anti-Mouse CD19 Alexa Fluor (R) 647 and 1.0 micrograms conjugated anti-Mouse CD20 Purified followed by Anti-Rat IgG PE. Quadrant lines represent Rat IgG2a isotype control staining levels and cells in the lymphocyte gate were used for analysis.
(3 Publications)
Species: Hu, Mu
Applications: WB, Flow
PLEKHM1 Antibody
NBP1-83013
Western Blot: PLEKHM1 Antibody [NBP1-83013] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Negative control (vector only transfected HEK293T lysate)Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)
Immunohistochemistry-Paraffin: PLEKHM1 Antibody [NBP1-83013] - Staining of human kidney shows strong cytoplasmic positivity in cells of tubules.
Species: Hu
Applications: WB, IHC, IHC-P
Nrf1 Antibody
NBP2-19586
Western Blot: Nrf1 Antibody [NBP2-19586] - A. 30 ug 293T whole cell lysate/extractB. 30 ug 293T nuclear lysate/extract C. 30 ug A431 whole cell lysate/extract | D. 30 ug A431 nuclear lysate/extract E. 30 ug HeLa whole cell lysate/extract F. 30 ug HeLa nuclear lysate/extract G. 30 ug HepG2 whole cell lysate/extract H. 30 ug HepG2 nuclear lysate/extract10 % SDS-PAGE NRF1 antibody dilution: 1:1000
Immunocytochemistry/Immunofluorescence: Nrf1 Antibody [NBP2-19586] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NRF1 protein stained by NRF1 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um
Immunohistochemistry-Paraffin: Nrf1 Antibody [NBP2-19586] - Paraffin-embedded mouse colon. NRF1 antibody dilution: 1:500.
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
mtRNA polymerase
Antibody
NBP2-19436
Western Blot: mtRNA polymerase Antibody [NBP2-19436] - Sample (30 ug of whole cell lysate) A: Hep G2 5% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: mtRNA polymerase Antibody [NBP2-19436] - Immunofluorescence analysis of methanol-fixed Hep G2, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: mtRNA polymerase Antibody [NBP2-19436] - Immunohistochemical analysis of paraffin-embedded Hepatoma, using antibody at 1:500 dilution.
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
MTERF Antibody
NBP1-92144
Western Blot: MTERF Antibody [NBP1-92144] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: MTERF Antibody [NBP1-92144] - Staining of human urinary bladder shows moderate cytoplasmic positivity in urothelial cells.
Species: Hu
Applications: WB, IHC, IHC-P
PPRC1 Antibody
NBP1-82985
Western Blot: PPRC1 Antibody [NBP1-82985] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue
Immunocytochemistry/Immunofluorescence: PPRC1 Antibody [NBP1-82985] - Staining of human cell line A-431 shows positivity in nucleus but not nucleoli. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PPRC1 Antibody [NBP1-82985] - Staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
GTF2H1 Antibody
NBP2-38556
Western Blot: GTF2H1 Antibody [NBP2-38556] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG
Immunocytochemistry/Immunofluorescence: GTF2H1 Antibody [NBP2-38556] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: GTF2H1 Antibody [NBP2-38556] - Staining of human testis shows high expression.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Cytochrome c
Antibody (7H8.2C12)
NB100-56503
Western Blot: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - Analysis using the Biotin conjugate of NB100-56503. Detection of Cytochrome C in 15 mgs of HeLa cell lysate using NB100-55775 at 1:1000. A 15 kDa band is detected.
Immunocytochemistry/Immunofluorescence: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - p73 was detected in immersion fixed Hela human cell line using Cytochrome C [7H8.2C12] Monoclonal Antibody (Catalog # NB100-56503) at 25 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Staining was observed in the cytoplasm and mitochondria.
Immunohistochemistry-Paraffin: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - Cytochrome C was detected in immersion fixed paraffin-embedded sections of human heart using anti-human mouse monoclonal antibody (Catalog # NB100-56503) at 1:200 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue).Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.
(47 Publications)
Species: Hu, Mu, Rt, Po, Ca, Dr, Eq, Ma, Rb
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, Flow-IC
Nrf2 Antibody
NBP1-32822
Western Blot: Nrf2 Antibody [NBP1-32822] - Non-transfected (-) and NRF2-transfected (+, including 3xFlag-tag) 293T whole cell extracts (30ug) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody diluted by 1:1000.
Immunocytochemistry/Immunofluorescence: Nrf2 Antibody [NBP1-32822] - NIH/3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NRF2 protein stained by NRF2 antibody [N2C2], Internal diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.
Immunohistochemistry-Paraffin: Nrf2 Antibody [NBP1-32822] - Paraffin-embedded Cal27 xenograft, using Nrf2 antibody at 1:500 dilution.
(3 Reviews)
(4 Publications)
Species: Hu, Mu, Rt, Av, Ce
Applications: WB, ChIP, ICC/IF, IHC, IHC-P, IP, ChIP
GTF2H4 Antibody
H00002968-B01P
Western Blot: GTF2H4 Antibody [H00002968-B01P] - Analysis of GTF2H4 expression in transfected 293T cell line by GTF2H4 polyclonal antibody. Lane 1: GTF2H4 transfected lysate(50.82 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/Immunofluorescence: GTF2H4 Antibody [H00002968-B01P] - Analysis of purified antibody to GTF2H4 on HeLa cell. (antibody concentration 10 ug/ml)
Species: Hu
Applications: WB, ICC/IF
PGC1 alpha Antibody
NBP1-04676
Western Blot: PGC1 alpha Antibody [NBP1-04676] - Total protein from human adipose and skeletal muscle tissue, HeLa and A431 cells lines was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-PGC1-alpha in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence
Immunohistochemistry-Paraffin: PGC1 alpha Antibody [NBP1-04676] - IHC analysis of PGC-1 alpha in mouse prostate using DAB with hematoxylin counterstain.
Immunohistochemistry-Paraffin: PGC1 alpha Antibody [NBP1-04676] - IHC analysis of a formalin-fixed paraffin-embedded tissue section of mouse prostate using rabbit polyclonal PGC1 alpha antibody at 1:200 dilution. The antibody generated an expected strong nuclear (punctate in some cells) and a weak cytoplasmic staining in the glandular cells lining of tubule-alveolar gland.
(10 Reviews)
(44 Publications)
Species: Hu, Mu, Rt, Ha, Sq
Applications: WB, ChIP, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
TFB1M Antibody
NBP1-51978
TFB1M Antibody
Species: Hu
Applications: WB, PEP-ELISA

Publications for TFB1M Antibody (NBP1-51978)(1)


Reviews for TFB1M Antibody (NBP1-51978) (0)

There are no reviews for TFB1M Antibody (NBP1-51978). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for TFB1M Antibody (NBP1-51978) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional TFB1M Products

Bioinformatics Tool for TFB1M Antibody (NBP1-51978)

Discover related pathways, diseases and genes to TFB1M Antibody (NBP1-51978). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for TFB1M Antibody (NBP1-51978)

Discover more about diseases related to TFB1M Antibody (NBP1-51978).
 

Pathways for TFB1M Antibody (NBP1-51978)

View related products by pathway.

PTMs for TFB1M Antibody (NBP1-51978)

Learn more about PTMs related to TFB1M Antibody (NBP1-51978).
 

Research Areas for TFB1M Antibody (NBP1-51978)

Find related products by research area.

Blogs on TFB1M

There are no specific blogs for TFB1M, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our TFB1M Antibody and receive a gift card or discount.


Bioinformatics

Gene Symbol TFB1M
Entrez
Uniprot