TFB1M Antibody

Western Blot: TFB1M Antibody [NBP1-51978] - (0.3ug/ml) staining of A431 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

TFB1M Antibody Summary

• Immunogen: Peptide with sequence C-ELKRRKSKNEEKE corresponding to C-Terminus according to NP_057104.2.
• Epitope: C-ELKRRKSKNEEKE
• Predicted Species: Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TFB1M
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 38 kDa band observed in A431 lysates (calculated MW of 39.5 kDa band according to NP_057104.2).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TFB1M Antibody

• CGI75
• CGI-75
• dimethyladenosine transferase 1, mitochondrial
• EC 2.1.1
• EC 2.1.1.-
• h-mtTFB
• h-mtTFB1
• homolog of yeast mitochondrial transcription factor B
• hTFB1M
• Mitochondrial 12S rRNA dimethylase 1
• mitochondrial dimethyladenosine transferase 1
• Mitochondrial transcription factor B1
• mtTFB
• mtTFB1
• S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase 1
• transcription factor B1, mitochondrial

Background

The transcription of genes from mitochondrial DNA requires a mitochondrial RNA polymerase (see POLRMT, MIM 601778) and a DNA-binding transcription factor (see TFAM, MIM 600438). Transcription factor B1 (TFB1M) is a part of this transcription complex.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TFB1M Antibody (NBP1-51978)(1)

Publication using NBP1-51978

• Sanchez-Ferrero E, Coto E, Blazquez M et al. Mutational screening of the Mitochondrial transcription factors B1 and B2 (TFB1M and TFB2M) in Parkinson's disease. Parkinsonism Relat Disord 2008-11-01 [PMID: 18980857]

Bioinformatics

• Gene Symbol: TFB1M
• Entrez:
  • 51106(Human)
• Uniprot:
  • NP_057104.2(Human)