Image submitted by Rebecca Leylek at Stanford University.I used Jurkat cells and did an intracellular stain with a FoxP3 fix/perm protocol. I used a Ms anti-Rb secondary fluorescent antibody to detect the primary antibody. I was able to detect a positive signal that seems to be target-specific binding. The sample labeled “Tube_004” was stained with the anti-TEX2 primary antibody at a dilution of 1/100 (3µg/mL) as well as the secondary antibody. The sample labeled “Tube_005” was stained with a Rb IgG Isotope control (also at 3µg/mL) and the secondary antibody.
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