Western Blot: TERT Antibody [NB110-89471] - Detection of Telomerase reverse transcriptase in HeLa nuclear extracts using NB110-89471.
Immunocytochemistry/ Immunofluorescence: TERT Antibody [NB110-89471] - Antibody was tested in V6.5 mouse embryonic stem cells (cat# NBP1-41162) against Dylight 488. Actin and nuclei were counterstained against ...read more
This Telomerase reverse transcriptase antibody is useful in Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen at ~120 kDa. In ICC/IF, puncatate nuclear staining can be seen in V6.5 mouse embryonic stem cells (Catalog No. NBP1-41162). The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
120 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Canine reactivity reported in scientific literature (PMID: 27876576). Immunogen sequence has 84% homology to rat and bovine proteins.
Packaging, Storage & Formulations
Store at 4C. Do not freeze.
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
0.1% Sodium Azide
Immunogen affinity purified
Alternate Names for TERT Antibody
EST2Telomerase-associated protein 2
Telomerase catalytic subunit
telomerase reverse transcriptase
Telomerase reverse transcriptase (TERT) is a ribonucleoprotein enzyme essential for eukaryotic chromosomal termini replication that is active in progenitor as well as cancer cells, and stays inactive or show very low activity in normal somatic cells. TERT is the catalytic component of the holoenzyme complex (TERT, DKC1, WDR79/TCAB1, NOP10, NHP2, GAR1, TEP1, EST1A, POT1 and TERC) that implicates in telomeres elongation by acting as a reverse transcriptase adding simple sequence repeats to chromosome ends by copying a template sequence within RNA component of the enzyme. For executing its versatile functions, TERT can interact with HSP90A, PTGES3, HSPA1A, RAN, XPO1, PTPN11, NCL, SMARCA4, MCRS1, PIF1, PML and GNL3L. TERT modulates Wnt signaling and plays important roles in aging and anti-apoptosis. Telomerase activation has been implicated in cell immortalization/ carcinogenesis and defects in TERT are associated with susceptibilty to aplastic anemia, coronary artery disease (CAD), dyskeratosis congenita autosomal dominant type 2 (DKCA2), pulmonary fibrosis, and/or bone marrow failure, telomere-related, type 1 (PFBMFT1), dyskeratosis congenita autosomal recessive type 4 (DKCB4), susceptibility to pulmonary fibrosis idiopathic (IPF).
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for TERT Antibody (NB110-89471). (Showing 1 - 1 of 1 FAQs).
Can NB110-89471 be used to do IHC?
This product has not been tested for use in IHC however if you would like to test this you will be eligible for our Innovator's Reward Program. Under the terms of this reward we will give you a full credit for testing a product in a previously untested species and/or application regardless of positive or negative results, and submitting the data in the form of a review. Read more about our Innovators Reward Program.
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