TERT Antibody

Images

 
Western Blot: TERT Antibody [NB110-89471] - Detection of Telomerase reverse transcriptase in HeLa nuclear extracts using NB110-89471.
Immunocytochemistry/ Immunofluorescence: TERT Antibody [NB110-89471] - Antibody was tested in V6.5 mouse embryonic stem cells (cat# NBP1-41162) against Dylight 488. Actin and nuclei were counterstained against ...read more

Product Details

Summary
Reactivity Hu, Mu, CaSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

TERT Antibody Summary

Immunogen
Synthetic peptide made to an internal portion of human Telomerase reverse transcriptase (within residues 900-950). [Swiss-Prot# O14746]
Localization
Nuclear
Marker
Embryonic Stem Cell Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TERT
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:50-1:100
Application Notes
This Telomerase reverse transcriptase antibody is useful in Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen at ~120 kDa. In ICC/IF, puncatate nuclear staining can be seen in V6.5 mouse embryonic stem cells (Catalog No. NBP1-41162). The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
120 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
HeLa Lysate (NB820-89387)
TERT Lysate (NBL1-16817)
v6.5 Mouse embryonic stem cells (NBP1-41162)
Publications
Read Publications using
NB110-89471 in the following applications:

Reactivity Notes

Canine reactivity reported in scientific literature (PMID: 27876576). Immunogen sequence has 84% homology to rat and bovine proteins.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TERT Antibody

  • EST2Telomerase-associated protein 2
  • hEST2
  • TCS1EC 2.7.7.49
  • Telomerase catalytic subunit
  • telomerase reverse transcriptase
  • TERT
  • TP2HEST2
  • TRTEC 2.7.7

Background

Telomerase reverse transcriptase (TERT) is a ribonucleoprotein enzyme essential for eukaryotic chromosomal termini replication that is active in progenitor as well as cancer cells, and stays inactive or show very low activity in normal somatic cells. TERT is the catalytic component of the holoenzyme complex (TERT, DKC1, WDR79/TCAB1, NOP10, NHP2, GAR1, TEP1, EST1A, POT1 and TERC) that implicates in telomeres elongation by acting as a reverse transcriptase adding simple sequence repeats to chromosome ends by copying a template sequence within RNA component of the enzyme. For executing its versatile functions, TERT can interact with HSP90A, PTGES3, HSPA1A, RAN, XPO1, PTPN11, NCL, SMARCA4, MCRS1, PIF1, PML and GNL3L. TERT modulates Wnt signaling and plays important roles in aging and anti-apoptosis. Telomerase activation has been implicated in cell immortalization/ carcinogenesis and defects in TERT are associated with susceptibilty to aplastic anemia, coronary artery disease (CAD), dyskeratosis congenita autosomal dominant type 2 (DKCA2), pulmonary fibrosis, and/or bone marrow failure, telomere-related, type 1 (PFBMFT1), dyskeratosis congenita autosomal recessive type 4 (DKCB4), susceptibility to pulmonary fibrosis idiopathic (IPF).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Ca
Applications: WB, ICC/IF

Publications for TERT Antibody (NB110-89471)(4)

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TERT Antibody (NB110-89471). (Showing 1 - 1 of 1 FAQs).

  1. Can NB110-89471 be used to do IHC?
    • This product has not been tested for use in IHC however if you would like to test this you will be eligible for our Innovator's Reward Program. Under the terms of this reward we will give you a full credit for testing a product in a previously untested species and/or application regardless of positive or negative results, and submitting the data in the form of a review. Read more about our Innovators Reward Program.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

DyLight 405 Labeled NB110-89471V
FITC Labeled NB110-89471F

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Bioinformatics Tool for TERT Antibody (NB110-89471)

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Research Areas for TERT Antibody (NB110-89471)

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Blogs on TERT.

Reversing Cancer with Telomerase Reverse Transcriptase (TERT)
Telomerase reverse transcriptase (TERT) is a ribonucleoprotein enzyme essential for eukaryotic chromosomal termini replication. It is a useful marker as it is active only in progenitor and most cancer cells, but inactive or (active at very low activit...  Read full blog post.

Featured Product Citation using Novus' hTERT Antibody
In December 2009, researchers at the University of Southern California cited using Novus’ mouse monoclonal Telomerase Reverse Transcriptase Antibody (cat# NB100-317) [PMID: 19923445]. Specifically, Novus’ hTERT antibody was used for immunofluorescent...  Read full blog post.

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Bioinformatics

Gene Symbol TERT
Entrez
Uniprot