TDG Antibody (622)

Western Blot: TDG Antibody (622) [NBP2-43717] - RNAseq analysis showed that essential factors of the BER pathway were statistically significantly down-regulated (p<0.05) in 96-h induced Saos2- (i) and Li-Fraumeni- (ii) p21WAF1/Cip1 Tet-ON cells (see also Additional file 1: Figure S3 for specific real time RT-PCR validation). Note that although in Saos2- p21WAF1/Cip1 Tet-ON cells OGG1 expression was not found by RNAseq analysis, specific real-time RT-PCR and microarray analysis (see also Additional file 1: Figure S3) [9] confirmed its decreased expression. Selective immunoblots for APEX1, LIG3, TDG, and MUTY confirmed the specificity of the RNA analysis results. a-Tubulin served as loading control. Image collected and cropped by CiteAb from the following publication (genomebiology.biomedcentral.com/articles/10.1186/s13059-018-1401-9), licensed under a CC-BY license.

Western Blot: TDG Antibody (622) [NBP2-43717] - Analysis of 293T mock transfected (-) or transfected with a construct expressing human TDG. 10 % SDS-PAGE TDG antibody dilution: 1:10000.

• Reactivity: Hu, Bv
• Applications: WB
• Clone: 622
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

TDG Antibody (622) Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human TDG. The exact sequence is proprietary.
• Predicted Species: Bovine (91%). Backed by our 100% Guarantee.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TDG
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:5000-1:20000
• Theoretical MW: 46 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse (88%), Rat (84%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for TDG Antibody (622)

• EC 3.2.2.29
• TDG
• thymine-DNA glycosylaseG/T mismatch-specific thymine DNA glycosylase

Background

Thymine DNA glycosylase is encoded by this gene belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TDG Antibody (NBP2-43717)(1)

Publication using NBP2-43717

• Galanos P, Pappas G, Polyzos A et al. Mutational signatures reveal the role of RAD52 in p53-independent p21-driven genomic instability. Genome Biol. 2018-03-16 [PMID: 29548335] (WB)

Bioinformatics

• Gene Symbol: TDG