TCEAL1 Overexpression Lysate

Images

 
Western Blot: TCEAL1 Overexpression Lysate (Adult Normal) [NBL1-16758] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TCEAL1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

TCEAL1 Overexpression Lysate Summary

Description

TCEAL1 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC219873

Accession#: NM_004780

Protein Tag: C-MYC/DDK
Gene
TCEAL1

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
18.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for TCEAL1 Overexpression Lysate

  • Nuclear phosphoprotein p21/SIIR
  • p21
  • pp21
  • SIIRTCEA-like protein 1
  • transcription elongation factor A (SII)-like 1
  • transcription elongation factor A protein-like 1
  • Transcription elongation factor S-II protein-like 1

Background

This gene encodes a member of the transcription elongation factor A (SII)-like (TCEAL) gene family. Members of this family may function as nuclear phosphoproteins that modulate transcription in a promoter context-dependent manner. The encoded protein is similar to transcription elongation factor A/transcription factor SII and contains a zinc finger-like motif as well as a sequence related to the transcription factor SII Pol II-binding region. It may exert its effects via protein-protein interactions with other transcriptional regulators rather than via direct binding of DNA. Multiple family members are located on the X chromosome. Alternative splicing results in multiple transcript variants encoding a single isoform. [provided by RefSeq]. Transcript Variant: This variant (1) encodes the complete protein. Variants 1 through 3 encode the same isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-29463
Species: Pm, Hu, Pm, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NB200-103
Species: Hu, Mu, Rt, Xp(-), Ye
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
AF6897
Species: Hu, Mu
Applications: IHC, WB
NBP2-68858
Species: Hu
Applications: IHC, IHC-P
NB200-106
Species: Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, WB
H00003429-A01
Species: Hu
Applications: ELISA, IHC, IHC-Fr, WB
NBP2-47561
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-80616
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
NBP2-03198
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP1-90949
Species: Hu
Applications: IHC, IHC-P, WB
NBP2-00776
Species: Ca, Hu, Pm, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP2-32840
Species: Hu, Pm, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NB500-106
Species: Ch, Dr, Fi, Hu, Mar, Mu, Po, Pm, Rb, Rt, Ye, Ze
Applications: ChIP, ELISA, Flow, ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, IP, Simple Western, WB
AF1555
Species: Hu
Applications: WB
NB100-56098
Species: Ca, Hu, Mu, Rt
Applications: IHC, IHC-P, IP, WB
NBP1-28566
Species: Bv, Hu, Pm, Mu, Po, Rt
Applications: Func, IHC, IHC-Fr, IHC-P, WB
NB100-56598
Species: Hu, Mu
Applications: IHC, IHC-Fr, IHC-P, KD, WB
NBP1-31308
Species: Bv, Fe, Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, Simple Western, WB
MAB7557
Species: Hu
Applications: IHC, WB
NBL1-16758
Species: Hu
Applications: WB

Publications for TCEAL1 Lysate (NBL1-16758) (0)

There are no publications for TCEAL1 Lysate (NBL1-16758).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for TCEAL1 Lysate (NBL1-16758) (0)

There are no reviews for TCEAL1 Lysate (NBL1-16758). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for TCEAL1 Lysate (NBL1-16758) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional TCEAL1 Products

Bioinformatics Tool for TCEAL1 Lysate (NBL1-16758)

Discover related pathways, diseases and genes to TCEAL1 Lysate (NBL1-16758). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for TCEAL1 Lysate (NBL1-16758)

Discover more about diseases related to TCEAL1 Lysate (NBL1-16758).
 

Pathways for TCEAL1 Lysate (NBL1-16758)

View related products by pathway.

PTMs for TCEAL1 Lysate (NBL1-16758)

Learn more about PTMs related to TCEAL1 Lysate (NBL1-16758).
 

Research Areas for TCEAL1 Lysate (NBL1-16758)

Find related products by research area.

Blogs on TCEAL1

There are no specific blogs for TCEAL1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our TCEAL1 Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol TCEAL1