TC21/R-Ras2 Antibody

Western blot shows lysates of Rat-2 rat embryonic fibroblast cell line and CHP-100 human neuroblastoma cell line. PVDF membrane was probed with 2 µg/mL Goat Anti-Human/Rat TC21/R-Ras2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3605) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human R-Ras, TC21/R-Ras2, and M-Ras/R-Ras3 (2 ng/lane) were included. A specific band for TC21/R-Ras2 was detected at approximately 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

TC21/R-Ras2 was detected in immersion fixed paraffin-embedded sections of normal human placenta using Goat Anti-Human/Rat TC21/R-Ras2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3605) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

TC21/R-Ras2 Antibody Summary

• Immunogen: E. coli-derived recombinant human TC21/R-Ras2
  Met1-Phe204
  Accession # P62070
• Specificity: Detects human and rat TC21/R-Ras2 in Western blots. Does not detect recombinant human R-Ras or M-Ras (R‑Ras3).
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: RRAS2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TC21/R-Ras2 Antibody

• ras-related protein R-Ras2
• related RAS viral (r-ras) oncogene homolog 2
• RRAS2
• TC21
• TC21Ras-like protein TC21
• Teratocarcinoma oncogene

Background

Teratocarcinoma oncogene 21 (TC21), also known as R-Ras2, is a small GTP-binding protein of the Ras superfamily of GTPases. TC21 comprises an R-Ras subfamily with fellow members R-Ras and M-Ras (R-Ras3). Overexpression and activating mutations of TC21 have been reported in several tumor tissues and cell lines.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TC21/R-Ras2 Antibody (AF3605)(2)

Publications using AF3605

• Shannon W, Nicole B, Amanda P et al. R-Ras subfamily proteins elicit distinct physiologic effects and phosphoproteome alterations in neurofibromin-null MPNST cells. Cell Commun Signal. 2021-09-16 [PMID: 34530870]
• Shannon W, Nicole B, Amanda P et al. R-Ras subfamily proteins elicit distinct physiologic effects and phosphoproteome alterations in neurofibromin-null MPNST cells. Cell Commun Signal. 2021-09-16 [PMID: 34530870]

Bioinformatics

• Gene Symbol: RRAS2
• Uniprot:
  • P62070()