HAF109). For additional reference, recombinant human R-Ras, TC21/R-Ras2, and M-Ras/R-Ras3 (2 ng/lane) were included. A specific band for TC21/R-Ras2 was detected at approximately 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of Rat-2 rat embryonic fibroblast cell line and CHP-100 human neuroblastoma cell line. PVDF membrane was probed with 2 µg/mL Goat Anti-Human/Rat TC21/R-Ras2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3605) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human R-Ras, TC21/R-Ras2, and M-Ras/R-Ras3 (2 ng/lane) were included. A specific band for TC21/R-Ras2 was detected at approximately 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3605-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38639-25ul | |||
![Western Blot: TC21/R-Ras2 Antibody (2D3-4B8) [H00022800-M01] - Analysis of RRAS2 expression in transfected 293T cell line by RRAS2 monoclonal antibody (M01), clone 2D3-4B8.Lane 1: RRAS2 transfected lysate (Predicted MW: 23.4 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/TC21-R-Ras2-Antibody-2D3-4B8-Western-Blot-H00022800-M01-img0019.jpg "Western Blot: TC21/R-Ras2 Antibody (2D3-4B8) [H00022800-M01] - Analysis of RRAS2 expression in transfected 293T cell line by RRAS2 monoclonal antibody (M01), clone 2D3-4B8.Lane 1: RRAS2 transfected lysate (Predicted MW: 23.4 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: TC21/R-Ras2 Antibody (2D3-4B8) [H00022800-M01] - Analysis of monoclonal antibody to RRAS2 on A-431 cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/TC21-R-Ras2-Antibody-2D3-4B8-Immunocytochemistry-Immunofluorescence-H00022800-M01-img0010.jpg)
![Immunohistochemistry-Paraffin: TC21/R-Ras2 Antibody (2D3-4B8) [H00022800-M01] - Analysis of monoclonal antibody to RRAS2 on formalin-fixed paraffin-embedded human dysgerminoma tissue. Antibody concentration 5 ug/ml.](http://images.novusbio.com/fullsize/TC21-R-Ras2-Antibody-2D3-4B8-Immunohistochemistry-Paraffin-H00022800-M01-img0012.jpg)
![Western Blot: TC21/R-Ras2 Antibody (2D3-4B8) [H00022800-M01] - RRAS2 monoclonal antibody (M01), clone 2D3-4B8. Analysis of RRAS2 expression in HeLa.](http://images.novusbio.com/fullsize/TC21-R-Ras2-Antibody-2D3-4B8-Western-Blot-H00022800-M01-img0014.jpg)
![Western Blot: TC21/R-Ras2 Antibody (2D3-4B8) [H00022800-M01] - RRAS2 monoclonal antibody (M01), clone 2D3-4B8. Analysis of RRAS2 expression in PC-12.](http://images.novusbio.com/fullsize/TC21-R-Ras2-Antibody-2D3-4B8-Western-Blot-H00022800-M01-img0015.jpg)
![Western Blot: TC21/R-Ras2 Antibody (2D3-4B8) [H00022800-M01] - RRAS2 monoclonal antibody (M01), clone 2D3-4B8. Analysis of RRAS2 expression in Raw 264.7.](http://images.novusbio.com/fullsize/TC21-R-Ras2-Antibody-2D3-4B8-Western-Blot-H00022800-M01-img0016.jpg)
![Western Blot: TC21/R-Ras2 Antibody (2D3-4B8) [H00022800-M01] - RRAS2 monoclonal antibody (M01), clone 2D3-4B8 Analysis of RRAS2 expression in A-431.](http://images.novusbio.com/fullsize/TC21-R-Ras2-Antibody-2D3-4B8-Western-Blot-H00022800-M01-img0017.jpg)
![Western Blot: TC21/R-Ras2 Antibody (2D3-4B8) [H00022800-M01] - RRAS2 monoclonal antibody (M01), clone 2D3-4B8. Analysis of RRAS2 expression in NIH/3T3.](http://images.novusbio.com/fullsize/TC21-R-Ras2-Antibody-2D3-4B8-Western-Blot-H00022800-M01-img0018.jpg)
![ELISA: TC21/R-Ras2 Antibody (2D3-4B8) [H00022800-M01] - Detection limit for recombinant GST tagged RRAS2 is 1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/TC21-R-Ras2-Antibody-2D3-4B8-ELISA-H00022800-M01-img0020.jpg)
![Western Blot: TC21/R-Ras2 Antibody (2D3-4B8) [H00022800-M01] - Validation of R-Ras2 as the defatty-acylation target of SIRT6.(A) mRNA & protein levels of R-Ras2 in Sirt6 WT & KO MEFs. Image collected & cropped by CiteAb from the following publication (//elifesciences.org/articles/25158), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00022800-m01_mouse-monoclonal-tc21-r-ras2-antibody-2d3-4b8-310202415291973.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: TC21/R-Ras2 Antibody (2D3-4B8) [H00022800-M01] - Identification of defatty-acylation targets of SIRT6 that contribute to its function in cell proliferation.(A) Cell proliferation of Sirt6 KO MEFs stably expressing pCDH empty vector, SIRT6 WT, G60A, or H133Y. Cell proliferation was assayed & quantified using crystal violet staining. Values with error bars indicate mean ± s.d. of three biological replicates. * indicates p<0.05 & ‘ns’ indicates no statistical significance when comparing to Sirt6 KO MEFs with pCDH. (B) Schematic overview of the SILAC experiment to identify SIRT6 lysine defatty-acylation targets. (C) Data analysis & filter of SILAC results. (D) Effects of R-Ras2 knockdown on the proliferation of Sirt6 WT & KO MEFs. Values with error bars indicate mean ± s.d. of three biological replicates. *** indicates p<0.005 for comparing to Sirt6 KO MEFs with ctrl shRNA.DOI://dx.doi.org/10.7554/eLife.25158.003Schematic overview of the SILAC experiment to compare the protein abundance in Sirt6 WT & KO MEFs.DOI://dx.doi.org/10.7554/eLife.25158.004 Image collected & cropped by CiteAb from the following publication (//elifesciences.org/articles/25158), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00022800-m01_mouse-monoclonal-tc21-r-ras2-antibody-2d3-4b8-31020241548228.jpg)
CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # ![Western Blot: TC21/R-Ras2 Antibody [NBP1-32919] - Sample (30 ug of whole cell lysate) A: A431 12% SDS PAGE; antibody diluted at 1:5000.](http://images.novusbio.com/fullsize/TC21-R-Ras2-Antibody-Western-Blot-NBP1-32919-img0009.jpg "Western Blot: TC21/R-Ras2 Antibody [NBP1-32919] - Sample (30 ug of whole cell lysate) A: A431 12% SDS PAGE; antibody diluted at 1:5000.")
![Immunohistochemistry-Paraffin: TC21/R-Ras2 Antibody [NBP1-32919] - Paraffin-embedded DLD1 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/TC21-R-Ras2-Antibody-Immunohistochemistry-Paraffin-NBP1-32919-img0007.jpg)
![Western Blot: TC21/R-Ras2 Antibody [NBP1-32919] - Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE; antibody diluted at 1:5000.](http://images.novusbio.com/fullsize/TC21-R-Ras2-Antibody-Western-Blot-NBP1-32919-img0008.jpg)
![Immunohistochemistry: TC21/R-Ras2 Antibody [NBP1-32919] - TC21/R-Ras2 antibody [N1C3] detects TC21/R-Ras2 protein on whole-mount zebrafish embryos by immunohistochemical analysis.?<br /> Sample: Paraformaldehyde-fixed zebrafish embryos. <br /> TC21/R-Ras2 antibody [N1C3] (NBP1-32919) dilution: 1:200.](http://images.novusbio.com/fullsize/nbp1-32919_rabbit-polyclonal-tc21-r-ras2-antibody-9102024206336.jpg)
![Immunocytochemistry/Immunofluorescence: TC21/R-Ras2 Antibody (EM-50) [NBP2-62216] - Colocalization of R-Ras2/TC21 signal (red) and GFP signal (green) in COS cells transfected with R-Ras2/TC21-GFP construct, after fixation and permeabilization. R-Ras2/TC21 was detected using monoclonal antibody EM-50, purified.](http://images.novusbio.com/fullsize/TC21-R-Ras2-Antibody-EM-50-Immunocytochemistry-Immunofluorescence-NBP2-62216-img0001.jpg "Immunocytochemistry/Immunofluorescence: TC21/R-Ras2 Antibody (EM-50) [NBP2-62216] - Colocalization of R-Ras2/TC21 signal (red) and GFP signal (green) in COS cells transfected with R-Ras2/TC21-GFP construct, after fixation and permeabilization. R-Ras2/TC21 was detected using monoclonal antibody EM-50, purified.")
![Flow Cytometry: TC21/R-Ras2 Antibody (EM-50) [NBP2-62216] - Intracellular staining of R-Ras2/TC21 in HEK-293 R-Ras2-GFP transfectants using monoclonal antibody EM-50, purified / GAM-APC.](http://images.novusbio.com/fullsize/TC21-R-Ras2-Antibody-EM-50-Flow-Cytometry-NBP2-62216-img0003.jpg)
![Flow (Intracellular): TC21/R-Ras2 Antibody (EM-50) [NBP2-62216] - Intracellular staining of R-Ras2/TC21 in HEK-293 R-Ras2 transfectants using monoclonal antibody EM-50, PE.](http://images.novusbio.com/fullsize/TC21-R-Ras2-Antibody-EM-50-Flow-Intracellular-NBP2-62216-img0002.jpg)
![Immunohistochemistry-Paraffin: TC21/R-Ras2 Antibody [NBP2-38639] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.](http://images.novusbio.com/fullsize/TC21-R-Ras2-Antibody-Immunohistochemistry-Paraffin-NBP2-38639-img0002.jpg "Immunohistochemistry-Paraffin: TC21/R-Ras2 Antibody [NBP2-38639] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.")
![Western Blot: TC21/R-Ras2 Antibody [NBP2-81761] - Western blot analysis of TC21/R-Ras2 in Jurkat cell lysate with TC21/R-Ras2 antibody at (A) 1 and (B) 2 ug/mL](http://images.novusbio.com/fullsize/TC21-R-Ras2-Antibody-Western-Blot-NBP2-81761-img0001.jpg "Western Blot: TC21/R-Ras2 Antibody [NBP2-81761] - Western blot analysis of TC21/R-Ras2 in Jurkat cell lysate with TC21/R-Ras2 antibody at (A) 1 and (B) 2 ug/mL")
![Western Blot: RRAS2 Overexpression Lysate (Adult Normal) [NBP2-07469] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RRAS2.](http://images.novusbio.com/fullsize/RRAS2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-07469-img0001.jpg "Western Blot: RRAS2 Overexpression Lysate (Adult Normal) [NBP2-07469] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RRAS2.")
![SDS-Page: RRAS2 Protein [NBP1-30244] - RRAS2, 25.2 kDa (221aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE](http://images.novusbio.com/fullsize/RRAS2-Protein-SDS-Page-NBP1-30244-img0001.jpg "SDS-Page: RRAS2 Protein [NBP1-30244] - RRAS2, 25.2 kDa (221aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE")
