TBX2 Antibody (393720) [Unconjugated]

TBX2 was detected in immersion fixed MCF-7 human breast cancer cell line using Rat Anti-Human TBX2 Monoclonal Antibody (Catalog # MAB50401) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red, upper panel; Catalog # NL013) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: ICC/IF
• Clone: 393720
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

TBX2 Antibody (393720) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human TBX2
  Thr593-Arg702
  Accession # Q13207
• Specificity: Detects human TBX2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) TBX3, rhTBX6, or rhT‑bet is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: TBX2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TBX2 Antibody (393720) [Unconjugated]

• FLJ10169
• T-box 2
• T-box protein 2
• T-box transcription factor TBX2
• TBX2

Background

T-box protein 2 (TBX2) is a member of the T-box family of transcription factors and functions in the transcriptional regulation of genes required for mesoderm differentiation and limb bud pattern formation. The 74 kDa (predicted MW) nuclear protein, like other T-box family members, contains a T-domain which is involved in DNA binding and dimerization of the protein (aa 99‑277). Human TBX2 shows 96%, 95%, and 71% amino acid identity with dog, mouse, and Xenopus tropicalis TBX2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TBX2
• Uniprot:
  • Q13207()