TBCC Antibody (3D3) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: TBCC Antibody (3D3) [H00006903-M04] - Analysis of monoclonal antibody to TBCC on HeLa cell. Antibody concentration 10 ug/ml

Sandwich ELISA: TBCC Antibody (3D3) [H00006903-M04] - Detection limit for recombinant GST tagged TBCC is 0.3 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 3D3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

TBCC Antibody (3D3) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: TBCC (AAH20170.1, 1 a.a. ~ 346 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MESVSCSAAAVRTGDMESQRDLSLVPERLQRREQERQLEVERRKQKRQNQEVEKENSHFFVATFARERAAVEELLERAESVERLEEAASRLQGLQKLINDSVFFLAAYDLRQGQEALARLQAALAERRRGLQPKKRFAFKTRGKDAASSTKVDAAPGIPPAVESIQDSPLPKKAEGDLGPSWVCGFSNLESQVLEKRASELHQRDVLLTELSNCTVRLYGNPNTLRLTKAHSCKLLCGPVSTSVFLEDCSDCVLAVACQQLRIHSTKDTRIFLQVTSRAIVEDCSGIQFAPYTWSYPEIDKDFESSGLDRSKNNWNDVDDFNWLARDMASPNWSILPEEERNIQWD
• Specificity: TBCC - tubulin-specific chaperone c (3D3)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TBCC
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for TBCC Antibody (3D3) - Azide and BSA Free

• CFCtubulin-specific chaperone c
• tubulin folding cofactor C
• Tubulin-folding cofactor C

Background

Cofactor C is one of four proteins (cofactors A, D, E, and C) involved in the pathway leading to correctly folded beta-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing and stabilizing beta-tubulin intermediates in a quasi-native confirmation. Cofactor E binds to the cofactor D/beta-tubulin complex; interaction with cofactor C then causes the release of beta-tubulin polypeptides that are committed to the native state. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TBCC Antibody (H00006903-M04)(3)

Publications using H00006903-M04

• Garcia-Mayoral MF, Castano R, Fanarraga ML et al. The solution structure of the N-terminal domain of human tubulin binding cofactor C reveals a platform for tubulin interaction. PLoS One. 2011-10-18 [PMID: 22028797]
• Hage-Sleiman R, Herveau S, Matera EL et al. Silencing of tubulin binding cofactor C modifies microtubule dynamics and cell cycle distribution and enhances sensitivity to gemcitabine in breast cancer cells. Mol Cancer Ther. 2011-01-07 [PMID: 21216936]
• Hage-Sleiman R, Herveau S, Matera EL et al. Tubulin binding cofactor C (TBCC) suppresses tumor growth and enhances chemosensitivity in human breast cancer cells. BMC Cancer 10:135. 2010-04-12 [PMID: 20384997]

Bioinformatics

• Gene Symbol: TBCC
• Entrez:
  • 6903(Human)
• Uniprot:
  • AAH20170(Human)