TAS1R1 Overexpression Lysate (Native)


Western Blot: TAS1R1 Overexpression Lysate (Adult Normal) [NBL1-16705] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TAS1R1.

Product Details

Reactivity HuSpecies Glossary
Applications WB

Order Details

TAS1R1 Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC212370 and based on accession number NM_177540. The protein contains a C-terminal DDK tag.
Homo sapiens taste receptor, type 1, member 1 (TAS1R1), transcript variant 3, mRNA.


Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer

Lysate Details for TAS1R1

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for TAS1R1 Overexpression Lysate (Native)

  • GPR70
  • G-protein coupled receptor 70
  • seven transmembrane helix receptor
  • T1R1TR1G protein-coupled receptor 70
  • taste receptor type 1 member 1
  • taste receptor, type 1, member 1


The protein encoded by this gene is a G protein-coupled receptor and is a component of the heterodimeric amino acid taste receptor T1R1+3. The T1R1+3 receptor responds to L-amino acids but not to D-enantiomers or other compounds. Most amino acids that are perceived as sweet activate T1R1+3, and this activation is strictly dependent on an intact T1R1+3 heterodimer. Multiple transcript variants encoding several different isoforms have been found for this gene. Transcript Variant: This variant (3) lacks an alternate in-frame exon compared to variant 2, resulting in an isoform (c) which is shorter but has the same N- and C-termini compared to isoform b.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Gene Symbol TAS1R1