TAO Kinase 1 Knockout HeLa Cell Lysate

Product Discontinued

TAO Kinase 1 Knockout HeLa Cell Lysate Summary

• Preparation Method: Knockout achieved by using CRISPR/Cas9,16 bp deletion in exon 2
• Gene: TAOK1

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: You will receive 1 vial (100ug) of knockout cell lysate and 1 vial (100ug) of Parental cell lysate. Lysate can be diluted with 1X SDS sample buffer and will be stable at -20 degrees C for 12 months. Minimize freeze-thaw cycles.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1 mg cell homogenate lyophilized in RIPA buffer made with double-knockout cell lines.
• Concentration: LYOPH
• Reconstitution Instructions: To use as WB negative control, spin down briefly and resuspend in 100 uL 1xSDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel.

Lysate Details for Array

• Type: Knockout HeLa Cell

Notes

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Validation of antibody specificity is critical and verification of antibody performance against knockout samples is one way to guarantee that an antibody recognizes a specific target. Novus' KO cell lysate can be used as a negative control for western blots and to confirm the specificity of antibodies.

Alternate Names for TAO Kinase 1 Knockout HeLa Cell Lysate

• EC 2.7.11
• FLJ14314
• hKFC-B
• hTAOK1
• KIAA1361
• KIAA1361STE20-like kinase PSK2
• Kinase from chicken homolog B
• MAP3K16
• MAP3K16EC 2.7.11.1
• MARK Kinase
• MARKK
• MARKKmicrotubule affinity regulating kinase kinase
• PSK2
• PSK-2
• PSK2serine/threonine kinase TAO1
• serine/threonine-protein kinase TAO1
• TAO Kinase 1
• TAO1serine/threonine protein kinase TAO1 homolog
• TAOK1
• Thousand and one amino acid protein 1

Background

TAOK1, a member of the Ste20 kinase family, is a TAO type protein kinase that acts as an upstream activator to MARK by phosphorylation. When activated, MARK enhances microtubule dynamics and leads to phosphorylation and detachment of tau or equivalent MAPs from microtubules. Overexpression of MARK eventually leads to microtubule breakdown and cell death, but in neuronal cells the primary effect is to allow the development of neurites during differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: TAOK1