TAO Kinase 1 Overexpression Lysate

Images

 
Western Blot: TAO Kinase 1 Overexpression Lysate (Adult Normal) [NBP2-06605] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TAO Kinase 1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

TAO Kinase 1 Overexpression Lysate Summary

Description

TAO Kinase 1 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC219490

Accession#: NM_020791

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
TAOK1

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
115.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for TAO Kinase 1 Overexpression Lysate

  • EC 2.7.11
  • FLJ14314
  • hKFC-B
  • hTAOK1
  • KIAA1361
  • KIAA1361STE20-like kinase PSK2
  • Kinase from chicken homolog B
  • MAP3K16
  • MAP3K16EC 2.7.11.1
  • MARK Kinase
  • MARKK
  • MARKKmicrotubule affinity regulating kinase kinase
  • PSK2
  • PSK-2
  • PSK2serine/threonine kinase TAO1
  • serine/threonine-protein kinase TAO1
  • TAO Kinase 1
  • TAO1serine/threonine protein kinase TAO1 homolog
  • TAOK1
  • Thousand and one amino acid protein 1

Background

TAOK1, a member of the Ste20 kinase family, is a TAO type protein kinase that acts as an upstream activator to MARK by phosphorylation. When activated, MARK enhances microtubule dynamics and leads to phosphorylation and detachment of tau or equivalent MAPs from microtubules. Overexpression of MARK eventually leads to microtubule breakdown and cell death, but in neuronal cells the primary effect is to allow the development of neurites during differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Video Protocols

WB Video Protocol

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Bioinformatics

Gene Symbol TAOK1