Tankyrase binding protein 1 Antibody

Western Blot: Tankyrase binding protein 1 Antibody [NB100-68249] - Detection of Human TAB182 by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TAB182 antibody NB100-68249 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunoprecipitation: Tankyrase binding protein 1 Antibody [NB100-68249] - Detection of human TAB182 (Tankyrase binding protein 1) by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TAB182 antibody NB100-68249 used for IP at 6 ug per reaction. TAB182 was also immunoprecipitated by rabbit anti-TAB182 antibodies NB100-68247 and NB100-68248. For blotting immunoprecipitated TAB182, NB100-68249 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Tankyrase binding protein 1 Antibody Summary

• Description: Novus Biologicals Rabbit Tankyrase binding protein 1 Antibody (NB100-68249) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1679 and 1729 of human tankyrase 1-binding protein of 182 kDa using the numbering given in entry NP_203754.2 (GeneID 85456).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TNKS1BP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-10 ug/mg lysate
  • Western Blot 1:2000-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Tankyrase binding protein 1 Antibody

• KIAA1741TAB182FLJ45975,182 kDa tankyrase-1-binding protein
• tankyrase 1 binding protein 1, 182kDa
• tankyrase 1-binding protein of 182 kDa

Background

TAB182 (tankyrase 1-binding protein of 182 kDa) was identified in a two-hybrid screen in search of tankyrase 1-interacting proteins. Tankyrase 1 is a poly (ADP-ribose) polymerase (PARP) that ribosylates the negative regulator of telomere length, TRF1 (telomere-repeat binding factor). Ribosylation of TRF1 has been shown to result in the inhibition of TRF1 binding and an induction of telomere elongation. TAB182 and TRF1 bind multiple discrete and overlapping sites of the tankyrase 1 ankyrin domain. This finding suggests that TAB182 may act as a scaffold to mediate higher order protein complex formation at telomeres. TAB182 has also been suggested to serve a role in the cytoplasm as it has been found to localize to the cytoplasmic cortical actin network.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TNKS1BP1
• Entrez:
  • 85456(Human)
• Uniprot:
  • NP_203754.2(Human)