TAF8 Antibody

Product Discontinued

TAF8 Antibody Summary

• Immunogen: Peptide with sequence KSYCEHTARTQPT corresponding to internal region according to NP_612639.2.
• Epitope: internal region
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TAF8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
• Application Notes: WB: Preliminary experiments gave an approx. 38 kDa band in human brain (amygdala, cerebellum, hippocampus) lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 34.3 kDa band according to NP_612639.2. The 38 kDa band was successfully blocked by incubation with the immunizing peptide.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for TAF8 Antibody

• 45/50kDa
• FLJ32821
• II
• Protein taube nuss
• RNA polymerase II, 43 kD
• TAF(II)43,43
• TAF8 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 43kDa
• TATA box binding protein (TBP)-associated factor, RNA polymerase II, A
• taube nuss homolog (mouse)
• TBP-associated factor 43 kDa
• TBP-associated factor 8
• transcription initiation factor TFIID subunit 8

Background

TAF8 encodes one of several TATA-binding protein (TBP)-associated factors (TAFs), which are integral subunits of the general transcription factor complex TFIID. TFIID recognizes the core promoter of many genes and nucleates the assembly of a transcription preinitiation complex containing RNA polymerase II and other initiation factors. The protein encoded by this gene contains an H4-like histone fold domain, and interacts with several subunits of TFIID including TBP and the histone-fold protein TAF10. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TAF8 Antibody (NBP1-46131)(1)

Publication using NBP1-46131

• Guermah M, Ge K, Chiang CM, Roeder RG. The TBN protein, which is essential for early embryonic mouse development, is an inducible TAFII implicated in adipogenesis. Mol Cell 2003-10-01 [PMID: 14580349]

Bioinformatics

• Gene Symbol: TAF8
• Entrez:
  • 129685(Human)
  • 63856(Mouse)
  • 316216(Rat)
• Uniprot:
  • NP_612639.2(Human)