TAF2 Overexpression Lysate (Native)

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Western Blot: TAF2 Overexpression Lysate (Adult Normal) [NBL1-16686] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TAF2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

TAF2 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC213159 and based on accession number NM_003184. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens TAF2 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 150kDa (TAF2), mRNA.
Gene
TAF2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
136.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for TAF2

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for TAF2 Overexpression Lysate (Native)

  • 150 kDa cofactor of initiator function
  • CIF150TAFII-150
  • cofactor of initiator function, 150kD subunit
  • TAF(II)150
  • TAF2 RNA polymerase II, TATA box binding protein (TBP)-associated factor
  • TAF2B
  • TAFII150RNA polymerase II TBP-associated factor subunit B
  • TATA box binding protein (TBP)-associated factor, RNA polymerase II, B, 150kD
  • TBP-associated factor 150 kDa
  • Transcription initiation factor TFIID 150 kDa subunit
  • transcription initiation factor TFIID subunit 2

Background

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the larger subunits of TFIID that is stably associated with the TFIID complex. It contributes to interactions at and downstream of the transcription initiation site, interactions that help determine transcription complex response to activators. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for TAF2 Lysate (NBL1-16686) (0)

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Bioinformatics

Gene Symbol TAF2