TAF1B Overexpression Lysate

Images

 
Western Blot: TAF1B Overexpression Lysate (Adult Normal) [NBL1-16685] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TAF1B.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

TAF1B Overexpression Lysate Summary

Description
Transient overexpression lysate of TATA box binding protein (TBP)-associated factor, RNA polymerase I, B, 63kDa (TAF1B) The lysate was created in HEK293T cells, using Plasmid ID RC202142 and based on accession number NM_005680. The protein contains a C-MYC/DDK Tag.
Gene
TAF1B

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
68.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for TAF1B Overexpression Lysate

  • RAF1BTATA box binding protein (TBP)-associated factor, RNA polymerase I, B, 63kD
  • RAFI63MGC:9349
  • RNA polymerase I-specific TBP-associated factor 63 kDa
  • SL1SL1, 63kD subunit
  • TAFI63TBP-associated factor 1B
  • TATA box binding protein (TBP)-associated factor, RNA polymerase I, B, 63kDa
  • TATA box-binding protein-associated factor 1B
  • TATA box-binding protein-associated factor RNA polymerase I subunit B
  • Transcription initiation factor SL1/TIF-IB subunit B

Background

Initiation of transcription by RNA polymerase I requires the formation of a complex composed of the TATA-binding protein (TBP) and three TBP-associated factors (TAFs) specific for RNA polymerase I. This complex, known as SL1, binds to the core promoter of ribosomal RNA genes to position the polymerase properly and acts as a channel for regulatory signals. This gene encodes one of the SL1-specific TAFs. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for TAF1B Lysate (NBL1-16685) (0)

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Bioinformatics

Gene Symbol TAF1B