Human t-Plasminogen Activator/tPA ELISA Kit (Colorimetric)

ELISA: Tissue Plasminogen Activator ELISA Kit 96Tests [NBP1-91260]

Product Discontinued

Human t-Plasminogen Activator/tPA ELISA Kit (Colorimetric) Summary

• Description: This kit is an enzyme-linked immunosorbent assay for quantitative detection.
• Specificity: There was no crossreactivity detected, namely not with human PAI-1 (plasminogen activator inhibitor 1, maximum concentration tested 2000 pg/ml).
• Standard Curve Range: 15.6 - 1,000 pg/mL
• Sensitivity: 6 pg/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 6.5%
• Intra-Assay: CV% < 3.6%
• Spike Recovery: The unspiked serum was used as blank in these experiments. The recovery ranged from 77% to 109% with an overall mean recovery of 98%.
• Sample Volume: 10 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: PLAT

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.

Alternate Names for Human t-Plasminogen Activator/tPA ELISA Kit (Colorimetric)

• Alteplase
• DKFZp686I03148
• EC 3.4.21
• EC 3.4.21.68
• plasminogen activator, tissue type
• plasminogen activator, tissue
• PLAT
• Reteplase
• tissue plasminogen activator (t-PA)
• tissue-type plasminogen activator
• TPA
• T-PA
• tPlasminogen Activator
• t-Plasminogen Activator

Background

Tissue-type plasminogen activator (t-PA) is a serine protease which occurs in blood plasma, serum, other body fluids, tissues and conditioned media of certain cultured cells. It can convert the inactive proenzyme plasminogen to the active protease plasmin. Plasmin can degrade fibrin, the matrix of a blood clot in a process known as fibrinolysis, leading to dissolution of the clot. Furthermore plasminogen activation is implicated in metastatic spread of malignant cells and in tissue remodelling. Elevated levels of t-PA in serum were shown to occur in relation to retinopathy in type 1 diabetes mellitus. The major field of clinical interest is the field of diseases of the heart. t-PA plasma levels have been shown to be altered with the presence of transplant coronary artery disease in cardiac transplant recipients. A correlation between low serum levels of t-PA activity and thrombotic tendency has been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for t-Plasminogen Activator/tPA ELISA Kit (NBP1-91260). (Showing 1 - 2 of 2 FAQ).

1. I am looking for an ELISA kit that measures the active form of tPA, specifically the free unbound form in plasma. Is ELISA kit NBP1-91260 suitable?
   • Yes, this kit detects natural and recombinant human tPA and tPA complexed with PAI-1.
2. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: PLAT