t-Plasminogen Activator/tPA Antibody (T-1) - BSA Free

Product Discontinued

t-Plasminogen Activator/tPA Antibody (T-1) - BSA Free Summary

• Immunogen: Native human tPA
• Epitope: MON T-1 binds to the A-chain of tPA (3)
• Specificity: Specific for human tPA. No reaction is seen with human urokinase plasminogen activator (uPA) when tested by ELISA, immunoblotting and enzyme inhibition and no reaction with any other human plasma proteins is seen when tested by immunoblotting.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PLAT
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation
  • Western Blot 1:100-1:2000
• Application Notes: T-1 reacts with non-reduced tPA but not with reduced tPA in Western blotting. It inhibits the enzymatic activity of tPA. For immunohistochemistry applications T-1 can be used on frozen sections.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 10mM Phosphate (pH 7.4) and 0.14M NaCl
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A or G purified

Alternate Names for t-Plasminogen Activator/tPA Antibody (T-1) - BSA Free

• Alteplase
• DKFZp686I03148
• EC 3.4.21
• EC 3.4.21.68
• plasminogen activator, tissue type
• plasminogen activator, tissue
• PLAT
• Reteplase
• tissue plasminogen activator (t-PA)
• tissue-type plasminogen activator
• TPA
• T-PA
• tPlasminogen Activator
• t-Plasminogen Activator

Background

Tissue plasminogen activator (tPA) is a serine protease which converts the abundant proenzyme plasminogen to active plasmin. It plays a key role in thrombolysis (1) and several neurobiological processes (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for t-Plasminogen Activator/tPA Antibody (NBP1-05159). (Showing 1 - 1 of 1 FAQ).

1. I have just purchased one of your antibodies for human tPA (catalog # NBP1-05159). I also noticed this comment on you product sheet: "T-1 reacts with non-reduced tPA but not with reduced tPA in Western blotting. It inhibits the enzymatic activity of tPA. For immunohistochemistry applications T-1 can be used on frozen sections." Does that mean that the antibody will only recognized non-denatured protein? Or it will indeed recognize denatured protein, as long as it is not reduced. If so, I should assume that it will work by simply excluding DTT from the loading buffer. I truly hope this antibody does not work only for native gels.
   • This antibody should recognize the denatured, but non-reduced protein. So you are correct, just exclude DTT and you can proceed as normal.

Bioinformatics

• Gene Symbol: PLAT