t-Plasminogen Activator/tPA Antibody (AH81-00)

Immunocytochemistry/Immunofluorescence: t-Plasminogen Activator/tPA Antibody (AH81-00) [NBP2-67280] - Staining TPA in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: t-Plasminogen Activator/tPA Antibody (AH81-00) [NBP2-67280] - Analysis of paraffin-embedded mouse skeletal muscle tissue using anti-TPA Tubulin antibody. Counter stained with hematoxylin.

Immunocytochemistry/Immunofluorescence: t-Plasminogen Activator/tPA Antibody (AH81-00) [NBP2-67280] - Staining TPA in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: t-Plasminogen Activator/tPA Antibody (AH81-00) [NBP2-67280] - Staining TPA in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: t-Plasminogen Activator/tPA Antibody (AH81-00) [NBP2-67280] - Analysis of paraffin-embedded human liver tissue using anti-TPA antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: t-Plasminogen Activator/tPA Antibody (AH81-00) [NBP2-67280] - Analysis of paraffin-embedded mouse brain tissue using anti-TPA antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: t-Plasminogen Activator/tPA Antibody (AH81-00) [NBP2-67280] - Analysis of paraffin-embedded mouse liver tissue using anti-TPA antibody. Counter stained with hematoxylin.

Product Discontinued

t-Plasminogen Activator/tPA Antibody (AH81-00) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Full length protein
• Localization: Secreted.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PLAT
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:10000
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000-1:5000

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 1%BSA, 40%Glycerol
• Preservative: 0.2% Sodium Azide
• Concentration: 2 mg/ml
• Purity: Protein A purified

Alternate Names for t-Plasminogen Activator/tPA Antibody (AH81-00)

• Alteplase
• DKFZp686I03148
• EC 3.4.21
• EC 3.4.21.68
• plasminogen activator, tissue type
• plasminogen activator, tissue
• PLAT
• Reteplase
• tissue plasminogen activator (t-PA)
• tissue-type plasminogen activator
• tPA
• T-PA
• tPlasminogen Activator
• t-Plasminogen Activator

Background

Tissue plasminogen activator (tPA) is a serine protease which converts the abundant proenzyme plasminogen to active plasmin. It plays a key role in thrombolysis (1) and several neurobiological processes (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PLAT