Syntenin 1 Antibody (JE40-72)

Western Blot: Syntenin 1 Antibody (JE40-72) [NBP2-76873] - Western blot analysis of Syntenin on A549 cell using anti-Syntenin antibody at 1/2,000 dilution.

Immunocytochemistry/Immunofluorescence: Syntenin 1 Antibody (JE40-72) [NBP2-76873] - ICC staining Syntenin in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry: Syntenin 1 Antibody (JE40-72) [NBP2-76873] - Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Syntenin antibody. Counter stained with hematoxylin.

Flow Cytometry: Syntenin 1 Antibody (JE40-72) [NBP2-76873] - Flow cytometric analysis of A549 cells with Syntenin antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: Syntenin 1 Antibody (JE40-72) [NBP2-76873] - ICC staining Syntenin in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry: Syntenin 1 Antibody (JE40-72) [NBP2-76873] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Syntenin antibody. Counter stained with hematoxylin.

Product Discontinued

Syntenin 1 Antibody (JE40-72) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human Syntenin 1 aa 1-118 / 298. (SwissProt: O00560 Human)
• Localization: Cytoplasm. Cytoskeleton. Membrane. Nucleus. Secreted.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SDCBP
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:200
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:10-1:50
  • Western Blot 1:500-1:2000
• Theoretical MW: 32 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Syntenin 1 Antibody (JE40-72)

• MDA9
• MDA-9
• melanoma differentiation associated protein-9
• Melanoma differentiation-associated protein 9
• Pro-TGF-alpha cytoplasmic domain-interacting protein 18
• Scaffold protein Pbp1
• ST1
• SYCLTACIP18
• syndecan binding protein (syntenin)
• Syndecan-binding protein 1
• syntenin-1

Background

Syntenin 1 is encoded by this gene was initially identified as a molecule linking syndecan-mediated signaling to the cytoskeleton. The syntenin protein contains tandemly repeated PDZ domains that bind the cytoplasmic, C-terminal domains of a variety of transmembrane proteins. This protein may also affect cytoskeletal-membrane organization, cell adhesion, protein trafficking, and the activation of transcription factors. The protein is primarily localized to membrane-associated adherens junctions and focal adhesions but is also found at the endoplasmic reticulum and nucleus. Alternative splicing results in multiple transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SDCBP