Syntaxin 1A Antibody (HPC-1)

Product Discontinued

Syntaxin 1A Antibody (HPC-1) Summary

• Immunogen: Antibody generated from a hybridoma that was established by fusion of mouse myeloma cell NS1 with Balb/c mouse splenocyte immunized with the synaptosomal plasma membrane fraction from adult rat hippocampus.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: STX1A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Immunoprecipitation 2 ug/200 ul cell extract
  • Western Blot 0.1 ug/ml
• Application Notes: For IHC-Paraffin: Heat treatment is necessary; microwave oven; 2 times for 10 minutes each in 10 mM citrate buffer (pH 6.5).

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 50% Glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Syntaxin 1A Antibody (HPC-1)

• Brain Syntaxin
• HPC-1
• Neuron-specific antigen HPC-1
• p35-1
• STX1A
• STX1P35-1
• SYN1A
• syntaxin 1A (brain)
• Syntaxin 1A
• syntaxin-1A

Background

Synaptic vesicles store neurotransmitters that are released during calcium-regulated exocytosis. The specificity of neurotransmitter release requires the localization of both synaptic vesicles and calcium channels to the presynaptic active zone. Syntaxins function in this vesicle fusion process. Syntaxins also serve as a substrate for botulinum neurotoxin type C, a metalloprotease that blocks exocytosis and has high affinity for a molecular complex that includes the alpha-latrotoxin receptor (MIM 600565) which produces explosive exocytosis (Zhang et al., 1995 [PubMed 7622072]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: STX1A
• Entrez:
  • 6804(Human)
  • 20907(Mouse)
  • 116470(Rat)
• Uniprot:
  • P32851(Rat)