Syntaxin 1A Antibody (HPC-1)

Images

 
Western Blot: Syntaxin 1a Antibody (HPC-1) [NBP1-54534] - Western blot analysis of syntaxin-1 expression in mouse brain extracts (1) and PC12 cells (2) using Syntaxin 1a Antibody (HPC-1).
Immunohistochemistry-Paraffin: Syntaxin 1a Antibody (HPC-1) [NBP1-54534] - Immunohistochemical detection of syntaxin-1 on paraffin embedded section of a human spinal cord with NBP1-54534.
Immunoprecipitation: Syntaxin 1a Antibody (HPC-1) [NBP1-54534] - Immunoprecipitation of syntaxin-1 from mouse brain extracts with NBP1-54534 (1) or mouse IgG1 (2).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clone
HPC-1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Syntaxin 1A Antibody (HPC-1) Summary

Immunogen
Antibody generated from a hybridoma that was established by fusion of mouse myeloma cell NS1 with Balb/c mouse splenocyte immunized with the synaptosomal plasma membrane fraction from adult rat hippocampus.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
STX1A
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Immunoprecipitation 2 ug/200 ul cell extract
Application Notes
For IHC-Paraffin: Heat treatment is necessary; microwave oven; 2 times for 10 minutes each in 10 mM citrate buffer (pH 6.5).

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 50% Glycerol
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Syntaxin 1A Antibody (HPC-1)

  • Brain Syntaxin
  • HPC-1
  • Neuron-specific antigen HPC-1
  • p35-1
  • STX1A
  • STX1P35-1
  • SYN1A
  • syntaxin 1A (brain)
  • Syntaxin 1A
  • syntaxin-1A

Background

Synaptic vesicles store neurotransmitters that are released during calcium-regulated exocytosis. The specificity of neurotransmitter release requires the localization of both synaptic vesicles and calcium channels to the presynaptic active zone. Syntaxins function in this vesicle fusion process. Syntaxins also serve as a substrate for botulinum neurotoxin type C, a metalloprotease that blocks exocytosis and has high affinity for a molecular complex that includes the alpha-latrotoxin receptor (MIM 600565) which produces explosive exocytosis (Zhang et al., 1995 [PubMed 7622072]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol STX1A
Entrez
Uniprot