Syntaxin 12 Antibody [Unconjugated]

Syntaxin 12 was detected in immersion fixed paraffin-embedded sections of human brain using Goat Anti-Human/Mouse Syntaxin 12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6617) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Syntaxin 12 was detected in perfusion fixed frozen sections of mouse brain (hippocampus) using Goat Anti-Human/Mouse Syntaxin 12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6617) at 15 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm in neurons and synaptic vesicles. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

• Reactivity: Hu, Mu
• Applications: IHC
• Host: Goat
• Conjugate: Unconjugated

Syntaxin 12 Antibody [Unconjugated] Summary

• Immunogen: E.coli-derived recombinant human Syntaxin 12
  Met1-Gln200
  Accession # Q86Y82
• Specificity: Detects human Syntaxin 12 in direct ELISAs. Detects human and mouse Syntaxin 12 in immunohistochemistry.
• Source: N/A
• Isotype: IgG
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Syntaxin 12 Antibody [Unconjugated]

• MGC51957
• STX12
• STX13
• STX14
• Syntaxin 12
• syntaxin-12

Background

Syntaxin 12/STX12 is a 39 kDa member of the Syntaxin family. It is a SNARE that acts to regulate protein transport between late endosomes and the trans-Golgi network. Human STX12 is 276 amino acids (aa) in length, and is a type IV single-pass transmembrane protein. The cytoplasmic region extends from aa 1-248. A coiled-coil region extends from aa 33-131 within that region. From aa 178-240, there is a t-SNARE coiled-coil homology domain. The transmembrane region is located at 249-269, and a vesicular area is located at aa 270-276. Human STX12 shares 92% aa sequence identity with mouse STX12.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Syntaxin 12 Antibody (AF6617)(1)

Publication using AF6617

• Cook, AA;Leung, TCS;Rice, M;Nachman, M;Zadigue-Dube, É;Watt, AJ; Endosomal dysfunction contributes to cerebellar deficits in spinocerebellar ataxia type 6 eLife 2023-12-12 [PMID: 38084749] (IHC, Transgenic Mouse)

Bioinformatics

• Uniprot:
  • Q86Y82()