SYK Antibody

Product Discontinued

SYK Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 150-200 of Human Syk.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SYK
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Application Notes: Western blot (WB) analysis of SYK (F169) pAb in extracts from HepG2 cells.
  Immunohistochemistry (IHC) analyzes of SYK (F169) pAb in paraffin-embedded human prostate carcinoma tissue.
• Theoretical MW: 72 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

This antibody is reactive against Human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SYK Antibody

• DKFZp313N1010
• EC 2.7.10
• EC 2.7.10.2
• FLJ25043
• spleen tyrosine kinaseFLJ37489
• SYK
• tyrosine-protein kinase SYK

Background

The Src-related protein tyrosine kinases Lck p56 and Fyn p59 are critically involved in T cell antigen receptor (TCR)/CD3-triggered activation. T lymphocytes also express a second class of non-receptor protein tyrosine kinases which includes Syk p70 and ZAP p72. These kinases resemble the Src family protein tyrosine kinases in that they have a C-terminal catalytic domain, but differ in that they are characterized by two SH2 domains but no SH3 domains. Evidence for the involvement of the Syk/ZAP family proteins in T cell activation was suggested by the finding that Syk p72 kinase fused to the transmembrane and extracellular domains of CD7 and DC16, respectively, can induce complete T cell activation. In contrast, the ZAP p70 kinase was insufficient unless it was coaggregated with a Fyn p59-containing chimera, suggesting that regulation of ZAP p70 activity may require a functional interaction with Src family kinases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SYK
• Entrez:
  • 6850(Human)