SURF5 Overexpression Lysate (Native)

Images

 
Western Blot: SURF5 Overexpression Lysate (Adult Normal) [NBL1-12987] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SURF5.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

SURF5 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC208015 and based on accession number NM_181491. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens mediator complex subunit 22 (MED22), transcript variant c, mRNA.
Gene
MED22

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
16.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for SURF5

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for SURF5 Overexpression Lysate (Native)

  • Med24
  • mediator complex subunit 22SURF5surfeit 5
  • mediator of RNA polymerase II transcription subunit 22
  • MGC48682
  • surf-5
  • Surfeit locus protein 5

Background

This gene is located in the surfeit gene cluster, a group of very tightly linked housekeeping genes that do not share sequence similarity. The gene is oriented in a head-to-head fashion with RPL7A (SURF3) and the two genes share a bidirectional promoter. The encoded proteins are localized to the cytoplasm. Two alternative transcript variants encoding different isoforms have been identified for this gene. Transcript Variant: This variant (c) differs in the 3' UTR and coding region compared to variant b. The resulting isoform (c) is shorter and has a distinct C-terminus compared to isoform b.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol MED22