SUN1 Antibody (JG95-31)

Western Blot: SUN1 Antibody (JG95-31) [NBP2-75677] - Analysis of SUN1 on rat kidney tissue lysate using anti-SUN1 antibody at 1/2,000 dilution.

Immunocytochemistry/Immunofluorescence: SUN1 Antibody (JG95-31) [NBP2-75677] - Staining SUN1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: SUN1 Antibody (JG95-31) [NBP2-75677] - Analysis of paraffin-embedded human colon tissue using anti-SUN1 antibody. Counter stained with hematoxylin.

Flow Cytometry: SUN1 Antibody (JG95-31) [NBP2-75677] - Analysis of SH-SY-5Y cells with SUN1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunohistochemistry-Paraffin: SUN1 Antibody (JG95-31) [NBP2-75677] - Analysis of paraffin-embedded human breast tissue using anti-SUN1 antibody. Counter stained with hematoxylin.

Product Discontinued

SUN1 Antibody (JG95-31) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human SUN1 aa 90-240 / 812. (SwissProt: O94901 Human)
• Localization: Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SUN1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 90 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for SUN1 Antibody (JG95-31)

• KIAA0810UNC84AFLJ12407
• MGC176649
• Protein unc-84 homolog A
• Sad1 and UNC84 domain containing 1
• Sad1 unc-84 domain protein 1
• Sad1/unc-84 protein-like 1
• SUN domain-containing protein 1
• unc-84 homolog A (C. elegans)
• unc-84 homolog A

Background

This gene is a member of the unc-84 homolog family and encodes a nuclear nuclear envelope protein with an Unc84 (SUN) domain. The protein is involved in nuclear anchorage and migration. Several alternatively spliced transcript variants of this gene have been described; however, the full-length nature of some of these variants has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SUN1