SUMO Activating Enzyme E1 (SAE1) Antibody

Western Blot: SUMO Activating Enzyme E1 (SAE1) Antibody [H00010055-B01P] - Analysis of SAE1 expression in human stomach.

Immunocytochemistry/Immunofluorescence: SUMO Activating Enzyme E1 (SAE1) Antibody [H00010055-B01P] - Analysis of purified antibody to SAE1 on HeLa cell. (antibody concentration 10 ug/ml)

Western Blot: SUMO Activating Enzyme E1 (SAE1) Antibody [H00010055-B01P] - Analysis of SAE1 expression in transfected 293T cell line by SAE1 polyclonal antibody. Lane 1: SAE1 transfected lysate(38.06 KDa). Lane 2: Non-transfected lysate.

Product Discontinued

SUMO Activating Enzyme E1 (SAE1) Antibody Summary

• Immunogen: SAE1 (NP_005491.1, 1 a.a. - 346 a.a.) full-length human protein. MVEKEEAGGGISEEEAAQYDRQIRLWGLEAQKRLRASRVLLVGLKGLGAEIAKNLILAGVKGLTMLDHEQVTPEDPGAQFLIRTGSVGRNRAEASLERAQNLNPMVDVKVDTEDIEKKPESFFTQFDAVCLTCCSRDVIVKVDQICHKNSIKFFTGDVFGYHGYTFANLGEHEFVEEKTKVAKVSQGVEDGPDTKRAKLDSSETTMVKKKVVFCPVKEALEVDWSSEKAKAALKRTTSDYFLLQVLLKFRTDKGRDPSSDTYEEDSELLLQIRNDVLDSLGISPDLLPEDFVRYCFSEMAPVCAVVGGILAQEIVKALSQRDPPHNNFFFFDGMKGNGIVECLGPK
• Specificity: SAE1 - SUMO1 activating enzyme subunit 1,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: SAE1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500
• Application Notes: Antibody reactive against Recombinant Protein with GST tag on ELISA and Western Blot and also on transfected lysate in western blot. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SUMO Activating Enzyme E1 (SAE1) Antibody

• AOS1
• AOS1activator of SUMO1
• FLJ3091
• HSPC140
• SAE1
• SUA1
• SUA1sentrin/SUMO-activating protein AOS1
• SUMO Activating Enzyme E1 (SAE1/UBA2)
• SUMO-1 activating enzyme E1 N subunit
• SUMO1 activating enzyme subunit 1
• SUMO-1 activating enzyme subunit 1
• SUMO-activating enzyme subunit 1
• UBA2
• Ubiquitin-like 1-activating enzyme E1A
• ubiquitin-like protein SUMO-1 activating enzyme
• UBLE1A

Background

The dimeric enzyme acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved cysteine residue on SAE2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SAE1
• Entrez:
  • 10055(Human)
• Uniprot:
  • NP_005491.1(Human)