SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11)

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Western Blot: SUMO Activating Enzyme E1 (SAE1) Antibody (6B11) [NBP2-45683] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SUMO Activating Enzyme E1.
Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (6B11) [NBP2-45683] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (6B11) [NBP2-45683] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (6B11) [NBP2-45683] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (6B11) [NBP2-45683] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (6B11) [NBP2-45683] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (6B11) [NBP2-45683] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (6B11) [NBP2-45683] - Analysis of Human lung tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (6B11) [NBP2-45683] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (6B11) [NBP2-45683] - Analysis of Human Ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (6B11) [NBP2-45683] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (6B11) [NBP2-45683] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (6B11) [NBP2-45683] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (6B11) [NBP2-45683] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (6B11) [NBP2-45683] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (6B11) [NBP2-45683] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (6B11) [NBP2-45683] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for ...read more
Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (6B11) [NBP2-45683] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (6B11) [NBP2-45683] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (6B11) [NBP2-45683] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (6B11) [NBP2-45683] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (6B11) [NBP2-45683] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (6B11) [NBP2-45683] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
OTI6B11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11) Summary

Immunogen
Full length human recombinant protein of human SAE1 (NP_005491) produced in HEK293T cell.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
SAE1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Western Blot 1:2000
Theoretical MW
38.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11)

  • AOS1
  • AOS1activator of SUMO1
  • FLJ3091
  • HSPC140
  • SAE1
  • SUA1
  • SUA1sentrin/SUMO-activating protein AOS1
  • SUMO Activating Enzyme E1 (SAE1/UBA2)
  • SUMO-1 activating enzyme E1 N subunit
  • SUMO1 activating enzyme subunit 1
  • SUMO-1 activating enzyme subunit 1
  • SUMO-activating enzyme subunit 1
  • UBA2
  • Ubiquitin-like 1-activating enzyme E1A
  • ubiquitin-like protein SUMO-1 activating enzyme
  • UBLE1A

Background

The dimeric enzyme acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved cysteine residue on SAE2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol SAE1