STI1 Antibody (JB95-38)

Western Blot: STI1 Antibody (JB95-38) [NBP2-75675] - Analysis of STIP1 on Mouse testis and Rat testis lysates using anti-STIP1 antibody at 1/500 dilution.

Immunohistochemistry-Paraffin: STI1 Antibody (JB95-38) [NBP2-75675] - Analysis of paraffin-embedded mouse testis tissue using anti-STIP1 antibody. Counter stained with hematoxylin.

Flow Cytometry: STI1 Antibody (JB95-38) [NBP2-75675] - Analysis of K562 cells with STIP1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunohistochemistry-Paraffin: STI1 Antibody (JB95-38) [NBP2-75675] - Analysis of paraffin-embedded human colon cancer tissue using anti-STIP1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: STI1 Antibody (JB95-38) [NBP2-75675] - Analysis of paraffin-embedded human spleen tissue using anti-STIP1 antibody. Counter stained with hematoxylin.

Product Discontinued

STI1 Antibody (JB95-38) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human STI1 aa 1-137 / 543. (SwissProt: P31948 Human; SwissProt: Q60864 Mouse; SwissProt: O35814 Rat)
• Localization: Cytoplasm, Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: STIP1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:500-1:1000
• Theoretical MW: 63 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for STI1 Antibody (JB95-38)

• HOP
• HOPSTI1L
• Hsc70/Hsp90-organizing protein
• Hsp70/Hsp90-organizing protein
• IEF-SSP-3521
• NY-REN-11 antigen
• NY-REN-11
• Renal carcinoma antigen NY-REN-11
• STI1
• STI1L
• STI1P60
• STIP1
• stress-induced-phosphoprotein 1
• Transformation-sensitive protein IEF SSP 3521

Background

STIP1 is an adaptor protein that coordinates the functions of HSP70 (see HSPA1A; MIM 140550) and HSP90 (see HSP90AA1; MIM 140571) in protein folding. It is thought to assist in the transfer of proteins from HSP70 to HSP90 by binding both HSP90 and substrate-bound HSP70. STIP1 also stimulates the ATPase activity of HSP70 and inhibits the ATPase activity of HSP90, suggesting that it regulates both the conformations and ATPase cycles of these chaperones (Song and Masison, 2005 (PubMed 16100115)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: STIP1