Stathmin-2/STMN2 Antibody (684433) [Unconjugated]

Western blot shows lysates of SH-SY5Y human neuroblastoma cell line. PVDF membrane was probed with 0.05 µg/mL of Mouse Anti-Human Stathmin-2/STMN2 Monoclonal Antibody (Catalog # MAB6930) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human Stathmin-1 and recombinant human Stathmin-2 (20 ng/lane) were included. A specific band was detected for Stathmin-2/STMN2 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western blot shows lysates of Neuro‑2A mouse neuroblastoma cell line, SH‑SY5Y human neuroblastoma cell line, Mouse Brain, and Rat Brain. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human/Mouse Stathmin‑2/STMN2 Monoclonal Antibody (Catalog # MAB6930) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Stathmin‑2/STMN2 at approximately 21-23 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Stathmin-2/STMN2 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Stathmin-2/STMN2 Monoclonal Antibody (Catalog # MAB6930) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clone: 684433
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Stathmin-2/STMN2 Antibody (684433) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Stathmin-2/STMN2
  Tyr26-Gly179
  Accession # Q93045
• Specificity: Detects human Stathmin-2/STMN2 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human Stathmin-1, -3, or -4 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: STMN2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 0.05 - 0.2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Stathmin-2/STMN2 Antibody (684433) [Unconjugated]

• FLJ34868
• FLJ50995
• neuron-specific growth-associated protein
• Protein SCG10
• SCG10
• SCG10neuronal growth-associated protein (silencer element)
• SCGN10
• SCGN10stathmin-2
• Stathmin2
• Stathmin-2
• stathmin-like 2
• STMN2
• superior cervical ganglia, neural specific 10
• Superior cervical ganglion-10 protein

Background

Stathmin-2 (STMN2), also known as superior cervical ganglion 10 protein (SCG10), is a 19 kDa microtubule-destabilizing protein that is preferentially expressed in neurons. It promotes neuronal proliferation and migration as well as neurite outgrowth. Stathmin-2 contains a membrane attachment region
(aa 1‑26), a regulatory domain (aa 39-96), and an overlapping coiled-coil domain (aa 75-179). It is subject to phosphorylation of multiple serines within the regulatory domain. Within aa 26‑179, human Stathmin-2 shares 100% and 99% aa sequence identity with mouse and rat Stathmin-2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Stathmin-2/STMN2 Antibody (MAB6930)(3)

Publications using MAB6930

• Sarah Pickles, Desiree Zanetti Alepuz, Yuka Koike, Mei Yue, Jimei Tong, Pinghu Liu et al. CRISPR interference to evaluate modifiers of C9ORF72-mediated toxicity in FTD Frontiers in Cell and Developmental Biology 2023-08-07 [PMID: 37614226]
• Thornburg-Suresh, EJC;Richardson, JE;Summers, DW; The Stathmin-2 membrane targeting domain is required for axon protection and regulated degradation by DLK signaling The Journal of biological chemistry 2023-05-24 [PMID: 37236359] (Western Blot, Human)
• Foster, SL;Dutton, AJ;Yerzhan, A;March, LB;Barry, K;Seehus, CR;Huang, X;Talbot, S;Woolf, CJ; A Preliminary Study of Mild Heat Stress on Inflammasome Activation in Murine Macrophages Cells 2023-04-19 [PMID: 37190098] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: STMN2
• Uniprot:
  • Q93045()