STAT2 Antibody (545117) [Unconjugated]

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, HeLa human cervical epithelial carcinoma cell line, and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.2 µg/mL of Human STAT2 Monoclonal Antibody (Catalog # MAB1666) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for STAT2 at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and STAT2 knockout HeLa cell line (KO). PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human STAT2 Monoclonal Antibody (Catalog # MAB1666) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for STAT2 at approximately 110 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Induction of type I IFN signaling by viral and nonviral stimuli is inhibited in G2/M-arrested cells. Suit2 cells were treated for 25 h with the vehicle, paclitaxel, colchicine, or ruxolitinib at 500 nM. Cells were then treated with the vehicle (untreated), TransIT reagent (0.5%, vol/vol), poly(I:C) at 10 μg/ml plus TransIT reagent, IFN-alpha at 5,000 U/ml, or VSV at an MOI of 30 based on titration on BHK-21 cells. VSV was aspirated 1 h later, and medium was added to infected wells. Cells remained in treatment for a total of 4 h, after which total protein was isolated. Western blot results for STAT1 and -2 proteins and their phosphorylated forms are shown in addition to VSV proteins. GAPDH was used to confirm that protein loading was the same across the gel. Protein names and protein sizes in kilodaltons are indicated on the left and right, respectively. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30487274), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB, KO
• Clone: 545117
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

STAT2 Antibody (545117) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human STAT2
  aa 679-851
  Accession # P52630
• Specificity: Detects endogenous human STAT2 in Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: STAT2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Knockout Validated
  • Western Blot 0.2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for STAT2 Antibody (545117) [Unconjugated]

• interferon alpha induced transcriptional activator
• ISGF-3
• MGC59816
• P113
• signal transducer and activator of transcription 2
• signal transducer and activator of transcription 2, 113kD
• signal transducer and activator of transcription 2, 113kDa
• STAT113
• STAT2

Background

Signal transducer and activator of transcription 2 (STAT2) is a member of the transcription factor STAT family with an apparent M.W. of 113 kDa in SDS-PAGE gels. Human STAT2 is 851 amino acids (aa) in length and contains one SH2 domain (aa 572-667). Splicing variants produce two isoforms for human STAT2. The short isoform has a 32 aa substitution and a 199 aa deletion corresponding to aa 621‑652 and aa 653‑851 in the long isoform, respectively. Human STAT2 shares 73% and 65% aa sequence identity with rat and mouse STAT2, in that order. STAT2 functions as a signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta ), and it is also required for myogenic differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for STAT2 Antibody (MAB1666)(2)

Publications using MAB1666

• PJ Metz, KA Ching, T Xie, P Delgado Cu, S Niessen, JH Tatlock, K Jensen-Per, BW Murray Symmetric Arginine Dimethylation Is Selectively Required for mRNA Splicing and the Initiation of Type I and Type III Interferon Signaling Cell Rep, 2020-02-11;30(6):1935-1950.e8. 2020-02-11 [PMID: 32049022] (Western Blot, Human)
• Christian Bressy, Gaith N. Droby, Bryant D. Maldonado, Nury Steuerwald, Valery Z. Grdzelishvili Cell Cycle Arrest in G 2 /M Phase Enhances Replication of Interferon-Sensitive Cytoplasmic RNA Viruses via Inhibition of Antiviral Gene Expression Journal of Virology 2019-02-15 [PMID: 30487274]

Bioinformatics

• Gene Symbol: STAT2
• Uniprot:
  • P52630()