STAT1 [p Ser727] Antibody (SN67-04)

Western Blot: STAT1 [p Ser727] Antibody (SN67-04) [NBP2-67657] - Analysis of STAT1 [p Ser727] on SiHa cell lysates. Lane 1: SiHa cells, whole cell lysate, 10 ug /lane. Lane 2: SiHa cells were treated with 2.8 ug/ul lambda-PP for 30 minutes, whole cell lysates, 10 ug/lane. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody Anti-STAT1 [p Ser727] (1/500) , Anti-STAT1 antibody (1/500) and Anti-HSP90 beta antibody (1/10,000)was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Predicted band size: 87 kDa Observed band size: 87 kDa Exposure time: 30 seconds

Immunohistochemistry-Paraffin: STAT1 [p Ser727] Antibody (SN67-04) [NBP2-67657] - Analysis of paraffin-embedded human breast invasive ductal tumor tissue with Rabbit anti-STAT1 [p Ser727] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Western Blot: STAT1 [p Ser727] Antibody (SN67-04) [NBP2-67657] - Western blot analysis of STAT1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rab

Immunohistochemistry-Paraffin: STAT1 [p Ser727] Antibody (SN67-04) [NBP2-67657] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-STAT1(S727) antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: STAT1 [p Ser727] Antibody (SN67-04) [NBP2-67657] - Analysis of paraffin-embedded human liver cancer tissue using anti-Phospho-STAT1(S727) antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: STAT1 [p Ser727] Antibody (SN67-04) [NBP2-67657] - Analysis of paraffin-embedded mouse colon tissue using anti-Phospho-STAT1(S727) antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: STAT1 [p Ser727] Antibody (SN67-04) [NBP2-67657] - Analysis of paraffin-embedded human liver carcinoma tissue with Rabbit anti-Phospho-STAT1 [p Ser727] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: STAT1 [p Ser727] Antibody (SN67-04) [NBP2-67657] - Analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-STAT1 [p Ser727] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: STAT1 [p Ser727] Antibody (SN67-04) [NBP2-67657] - Analysis of paraffin-embedded mouse colon tissue with Rabbit anti-STAT1 [p Ser727] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: STAT1 [p Ser727] Antibody (SN67-04) [NBP2-67657] - Analysis of paraffin-embedded rat colon tissue with Rabbit anti-Phospho-STAT1 [p Ser727] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Product Discontinued

STAT1 [p Ser727] Antibody (SN67-04) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser727 of Human STAT1 aa 701-750 / 750. (SwissProt: P42224 Human; SwissProt: P42225 Mouse; Entrez Gene: 25124 Rat)
• Modification: p Ser727
• Localization: Nucleus, Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: STAT1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for STAT1 [p Ser727] Antibody (SN67-04)

• CANDF7
• DKFZp686B04100
• ISGF-3
• ISGF-3,91kD
• signal transducer and activator of transcription 1, 91kDa
• signal transducer and activator of transcription-1
• STAT1
• STAT91

Background

STATs (signal transducers and activators of transcription) are a family of cytoplasmic latent transcription factors that are activated to regulate gene expression in response to a large number of extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STATs enter the nucleus to regulate transcription of many different genes. Among the seven STATs (Stat1, Stat2, Stat3, Stat4, Stat5a, Stat5b, and Stat6), Stat1, Stat3, Stat5a, and Stat5b have a wide activation profile. STAT1 is activated by many different ligands including IFN family (IFN-a, IFN-b, IFN-g and IL-10), gp130 family (IL-6, IL-11, LIF, CNTF, and G-CSF), and receptor tyrosine kinases (EGF, PDGF, and CSF-1). STAT1 has two forms, the 91 kDa STAT1a and the 84 kDa STAT1b which are encoded by the same gene with splicing variant.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: STAT1