SSX4 Antibody - BSA Free

Western Blot: SSX4 Antibody - BSA Free [NBP3-45811] - MCF7 cells were subjected to SDS PAGE followed by western blot with (SSX4 antibody) at dilution of 1:1000

Immunohistochemistry: SSX4 Antibody - BSA Free [NBP3-45811] - Immunohistochemistry of paraffin-embedded human testis using (SSX4 antibody) at dilution of 1:50

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SSX4 Antibody - BSA Free Summary

• Immunogen: synovial sarcoma, X breakpoint 4. (Uniprot# O60224)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SSX4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1:20-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 22 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% Glycerol, pH (7.3)
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for SSX4 Antibody - BSA Free

• Cancer/testis antigen 5.4
• CT5.4SSX4B
• MGC119056
• MGC12411
• protein SSX4
• SSX4A
• synovial sarcoma, X breakpoint 4

Background

The product of this gene belongs to the family of highly homologous synovial sarcoma X (SSX) breakpoint proteins. These proteins may function as transcriptional repressors. They are also capable of eliciting spontaneously humoral and cellular immune responses in cancer patients, and are potentially useful targets in cancer vaccine-based immunotherapy. SSX1, SSX2 and SSX4 genes have been involved in the t(X;18) translocation characteristically found in all synovial sarcomas. This translocation results in the fusion of the synovial sarcoma translocation gene on chromosome 18 to one of the SSX genes on chromosome X. Chromosome Xp11 contains a segmental duplication resulting in two identical copies of synovial sarcoma, X breakpoint 4, SSX4 and SSX4B, in tail-to-tail orientation. This gene, SSX4, represents the more telomeric copy. Two transcript variants encoding distinct isoforms have been identified for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SSX4