SSRP1 Antibody (10D1)

Product Discontinued

SSRP1 Antibody (10D1) Summary

• Immunogen: Recombinant (partial)
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SSRP1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation 1:10-1:500
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1 ug/ml
• Application Notes: ChIP (see Tan BC, et al.), IP and IF use reported in literature.
• Theoretical MW: 81 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for SSRP1 Antibody (10D1)

• Chromatin-specific transcription elongation factor 80 kDa subunit
• cisplatin-DNA SSRP
• facilitates chromatin remodeling 80 kDa subunit
• Facilitates chromatin transcription complex 80 kDa subunit
• Facilitates chromatin transcription complex subunit SSRP1
• FACT complex subunit SSRP1
• FACT
• FACT80FACT 80 kDa subunit
• FACTp80
• high mobility group box
• hSSRP1
• Recombination signal sequence recognition protein 1
• structure specific recognition protein 1
• Structure-specific recognition protein 1
• T160

Background

SSRP1 is a nuclear protein and a component of the FACT complex (facilitates chromatin transcription). The FACT complex is composed of the SUPT16H (FACT140) and the SSRP1 FACTp80 proteins. This complex interacts with nucleosomes and histone H2A/H2B dimers to promote nucleosome disassembly and allow transcription elongation. This protein interacts with dsDNA. The SSRP1 protein has been shown to be modified by cisplatin and may protect DNA from repair and block DNA replication. The 10D7 monoclonal antibody recognizes the human SSRP1 protein and has been shown to be useful for Western blotting.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SSRP1 Antibody (NB100-78323)(1)

Publication using NB100-78323

• Tan BC et al. EMBO 25:3975. 2006-01-01 [PMID: 16902406] (Chemotaxis)

Review for SSRP1 Antibody (NB100-78323) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 10/16/2014

Summary

• Application: Western Blot
• Sample Tested: WB for FACT160 in 293T cells
• Species: Human

Bioinformatics

• Gene Symbol: SSRP1
• Entrez:
  • 6749(Human)
• Uniprot:
  • Q08945(Human)