SSRP1 Antibody (10D1)

Images

 
Western Blot: SSRP1 Antibody (10D1) [NB100-78323] - analysis of SSRP1 in 293T cell lysate (30ug) using anti-SSRP1 antibody. Image from verified customer review.
Western Blot: SSRP1 Antibody (10D1) [NB100-78323] - la cell lysate was resolved by electrophoresis, transferred to nitrocellulose, and probed with monoclonal anti-SSRP1 antibody. Proteins were detected using a goat ...read more

Product Details

Summary
Product Discontinued
View other related SSRP1 Primary Antibodies

Order Details


    • Catalog Number
      NB100-78323
    • Availability
      Product Discontinued

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SSRP1 Antibody (10D1) Summary

Immunogen
Recombinant (partial)
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SSRP1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • Chromatin Immunoprecipitation 1:10-1:500
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 1:10-1:500
Application Notes
ChIP (see Tan BC, et al.), IP and IF use reported in literature.
Theoretical MW
81 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NB100-78323 in the following applications:

Publications
Read Publication using NB100-78323.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.2)
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for SSRP1 Antibody (10D1)

  • Chromatin-specific transcription elongation factor 80 kDa subunit
  • cisplatin-DNA SSRP
  • facilitates chromatin remodeling 80 kDa subunit
  • Facilitates chromatin transcription complex 80 kDa subunit
  • Facilitates chromatin transcription complex subunit SSRP1
  • FACT complex subunit SSRP1
  • FACT
  • FACT80FACT 80 kDa subunit
  • FACTp80
  • high mobility group box
  • hSSRP1
  • Recombination signal sequence recognition protein 1
  • structure specific recognition protein 1
  • Structure-specific recognition protein 1
  • T160

Background

SSRP1 is a nuclear protein and a component of the FACT complex (facilitates chromatin transcription). The FACT complex is composed of the SUPT16H (FACT140) and the SSRP1 FACTp80 proteins. This complex interacts with nucleosomes and histone H2A/H2B dimers to promote nucleosome disassembly and allow transcription elongation. This protein interacts with dsDNA. The SSRP1 protein has been shown to be modified by cisplatin and may protect DNA from repair and block DNA replication. The 10D7 monoclonal antibody recognizes the human SSRP1 protein and has been shown to be useful for Western blotting.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SSRP1 Antibody (NB100-78323)(1)

We have publications tested in 1 application: ChIP.


Filter By Application
ChIP
(1)
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Showing Publication 1 - 1 of 1.
Publication using NB100-78323 Applications Species
Tan BC et al. EMBO 25:3975. 2006 [PMID: 16902406] (ChIP) ChIP

Review for SSRP1 Antibody (NB100-78323) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB100-78323:
Filter by Applications
WB
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Western Blot SSRP1 NB100-78323
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reviewed by:
Anonymous
WB Human 10/16/2014
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Summary

ApplicationWestern Blot
Sample TestedWB for FACT160 in 293T cells
SpeciesHuman

Blocking

Blocking Details5% milk in TBST buffer

Primary Anitbody

Dilution Ratio1:1000, diluted in TBST buffer+5% milk

Secondary Antibody

Secondary Descriptiongoat anti-mouse IgG-HRP (SCBT)
Secondary Manufacturer Cat## sc-2031
Secondary Concentration1:5000

Details

Detection NotesDetected using Santa Cruz Western Blotting Luminol Reagent, Exposure 20 sec

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for SSRP1 Antibody (NB100-78323) (0)

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Secondary Antibodies

 

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Bioinformatics Tool for SSRP1 Antibody (NB100-78323)

Discover related pathways, diseases and genes to SSRP1 Antibody (NB100-78323). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for SSRP1 Antibody (NB100-78323)

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Pathways for SSRP1 Antibody (NB100-78323)

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Blogs on SSRP1

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Recent Reviews

5
5
1
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1
0

Anonymous
10/16/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol SSRP1
Entrez
Uniprot