SRPRB Antibody (OTI1D9)

Western Blot: SRPRB Antibody (1D9) [NBP2-02028] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SRPRB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SRPRB.

Immunohistochemistry-Paraffin: SRPRB Antibody (1D9) [NBP2-02028] Staining of paraffin-embedded Human tonsil using anti-SRPRB mouse monoclonal antibody.

Flow Cytometry: SRPRB Antibody (1D9) [NBP2-02028] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SRPRB antibody, and then analyzed by flow cytometry.

Immunohistochemistry-Paraffin: SRPRB Antibody (1D9) [NBP2-02028] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SRPRB mouse monoclonal antibody.

Immunohistochemistry-Paraffin: SRPRB Antibody (1D9) [NBP2-02028] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SRPRB mouse monoclonal antibody.

Immunohistochemistry-Paraffin: SRPRB Antibody (1D9) [NBP2-02028] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SRPRB mouse monoclonal antibody.

Immunohistochemistry-Paraffin: SRPRB Antibody (1D9) [NBP2-02028] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-SRPRB mouse monoclonal antibody.

Immunohistochemistry-Paraffin: SRPRB Antibody (1D9) [NBP2-02028] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SRPRB mouse monoclonal antibody.

Immunohistochemistry-Paraffin: SRPRB Antibody (1D9) [NBP2-02028] - Staining of paraffin-embedded Human colon tissue using anti-SRPRB mouse monoclonal antibody.

Immunohistochemistry-Paraffin: SRPRB Antibody (1D9) [NBP2-02028] - Staining of paraffin-embedded Human endometrium tissue using anti-SRPRB mouse monoclonal antibody.

Immunohistochemistry-Paraffin: SRPRB Antibody (1D9) [NBP2-02028] - Staining of paraffin-embedded Human pancreas tissue using anti-SRPRB mouse monoclonal antibody.

Immunohistochemistry-Paraffin: SRPRB Antibody (1D9) [NBP2-02028] - Staining of paraffin-embedded Human prostate tissue using anti-SRPRB mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, IHC
• Clone: OTI1D9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.88 mg/ml

SRPRB Antibody (OTI1D9) Summary

• Description: Novus Biologicals Mouse SRPRB Antibody (OTI1D9) (NBP2-02028) is a monoclonal antibody validated for use in IHC, WB, Flow and IP. Anti-SRPRB Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human SRPRB(NP_067026) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SRPRB
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:1000
• Theoretical MW: 29.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.88 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SRPRB Antibody (OTI1D9)

• APMCF1
• Protein APMCF1
• signal recognition particle receptor subunit beta
• signal recognition particle receptor, B subunit
• signal recognition particle receptor, beta subunit
• SR-beta

Background

The protein encoded by this gene has similarity to mouse protein which is a subunit of the signal recognition particle receptor (SR). This subunit is a transmembrane GTPase belonging to the GTPase superfamily. It anchors alpha subunit, a peripheral membrane GTPase, to the ER membrane. SR is required for the cotranslational targeting of both secretory and membrane proteins to the ER membrane. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SRPRB Antibody (NBP2-02028)(1)

Publication using NBP2-02028

• Park J, Chang J, Hwang HJ et al. The pioneer round of translation ensures proper targeting of ER and mitochondrial proteins Nucleic Acids Research 2021-12-02 [PMID: 34850140] (Immunoprecipitation, Western Blot)

Review for SRPRB Antibody (NBP2-02028) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 06/22/2016

Summary

• Application: Western Blot
• Species: Human

Bioinformatics

• Gene Symbol: SRPRB
• Entrez:
  • 58477(Human)