SRGAP2 Antibody

Images

 
Western Blot: SRGAP2 Antibody [NBP1-49877] - (0.1ug/ml) staining of Rat Cortical Neuron lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Data kindly provided by Ms. ...read more
Immunocytochemistry/ Immunofluorescence: SRGAP2 Antibody [NBP1-49877] - HEK293 overexpressing Human srGAP2 and probed with EB09775 at 2.5ug/ml in the right panel. Data kindly provided by Ms. Ya-Jing Mi and Dr.Wei-Lin ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

SRGAP2 Antibody Summary

Immunogen
Peptide with sequence C-KQEDRQTPRSPDST corresponding to internal region according to NP_056141.2.
Epitope
KQEDRQTPRSPDST - Internal region
Specificity
This antibody is expected to recognize isoform a (NP_056141.2).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SRGAP2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 - 0.3 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:2000
  • Peptide ELISA Detection limit 1:8000
Application Notes
IF: Transfected HEK293 transiently expressing GFP-tagged human srGAP2 with the primary antibody stained in the red channel (Confocal IF). WB: Approx. 124 kDa band observed in HeLa lysate and in rat primary culture cortical neurons at Day 4 (calculated MW of 121 kDa band according to human NP_056141.2, but the rat protein sequence is stlll provisional).
Publications
Read Publication using NBP1-49877.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for SRGAP2 Antibody

  • FNBP2
  • Formin-binding protein 2
  • KIAA0456ARHGAP34formin binding protein 2
  • Rho GTPase-activating protein 34
  • SLIT-ROBO Rho GTPase activating protein 2
  • SLIT-ROBO Rho GTPase-activating protein 2
  • srGAP2
  • SRGAP3

Background

SRGAP2 is a putative GTPase-activating protein for Rho family small GTPases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SRGAP2 Antibody (NBP1-49877)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SRGAP2
Entrez
Uniprot