SRGAP1 Antibody (5D10) - Azide and BSA Free

Western Blot: SRGAP1 Antibody (5D10) [H00057522-M07] - Analysis of SRGAP1 expression in IMR-32 (Cat # L008V1).

Immunocytochemistry/Immunofluorescence: SRGAP1 Antibody (5D10) [H00057522-M07] - Analysis of monoclonal antibody to SRGAP1 on HeLa cell. Antibody concentration 10 ug/ml

Western Blot: SRGAP1 Antibody (5D10) [H00057522-M07] - Analysis of SRGAP1 expression in NIH/3T3 (Cat # L018V1).

Sandwich ELISA: SRGAP1 Antibody (5D10) [H00057522-M07] - Detection limit for recombinant GST tagged SRGAP1 is 0.03 ng/ml as a capture antibody.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF
• Clone: 5D10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

SRGAP1 Antibody (5D10) - Azide and BSA Free Summary

• Immunogen: SRGAP1 (NP_065813, 952 a.a. ~ 1050 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PLDPETIAQDIEETMNTALNELRELERQSTAKHAPDVVLDTLEQVKNSPTPATSTESLSPLHNVALRSSEPQIRRSTSSSSDTMSTFKPMVAPRMGVQL
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SRGAP1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot
• Application Notes: This antibody is useful for ELISA, Western Blot

Reactivity Notes

This product is reactive against Human,Mouse.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SRGAP1 Antibody (5D10) - Azide and BSA Free

• ARHGAP13srGAP1
• FLJ22166
• KIAA1304SLIT-ROBO Rho GTPase-activating protein 1
• Rho GTPase-activating protein 13
• SLIT-ROBO Rho GTPase activating protein 1

Background

SRGAP1/ARHGAP13 is a member of the srGAP family of proteins that contain a RhoGAP domain, an SH3 domain, and a Fes/CIP4 homology domain and interact with the intracellular domain of roundabout 1 (Robo1). Robo1 is the receptor for Slit signaling and is involved in neuronal cell migration and axon tract positioning. SRGAP1/ARHGAP13 functions to bind and decrease the level of active Cdc42 and RhoA small GTPases during Slit-Robo signaling. Alternate names for SRGAP1/ARHGAP13 include Rho GTPase-activating protein 13, and KIAA1304.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SRGAP1 Antibody (H00057522-M07)(1)

Publication using H00057522-M07

• Hwang DY, Kohl S, Fan X et al. Mutations of the SLIT2-ROBO2 pathway genes SLIT2 and SRGAP1 confer risk for congenital anomalies of the kidney and urinary tract. Hum Genet. 2015-05-31 [PMID: 26026792]

Bioinformatics

• Gene Symbol: SRGAP1
• Uniprot:
  • NP_065813(Human)