Src Antibody (N6L)

Western Blot: Src Antibody (N6L) [NBP2-29829] - Representative lot data. A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Src, clone N6L (1:1,000 dilution). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Src (~ 60 kDa).

Product Discontinued

Src Antibody (N6L) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: N-terminal 6His-tagged recombinant full-length human Src expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.
• Specificity: Recognizes Src. Crossreacts with Yes and weakly with Fyn. No crossreactivity observed with Lck or Lyn.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SRC
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100-1:2000
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Tissue culture supernatant
• Preservative: 0.05% Sodium Azide
• Purity: Tissue culture supernatant

Notes

Relevant Entrez Gene Numbers: NP_005408.1

Alternate Names for Src Antibody (N6L)

• ASV
• c-Src
• EC 2.7.10
• EC 2.7.10.2
• pp60c-src
• Rous sarcoma
• RSVgp4
• Src
• tyrosine kinase pp60c-src
• tyrosine-protein kinase SRC-1
• v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog
• v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)

Background

pp60c-Src is the prototype non-receptor tyrosine kinase. Homology to Src in three regions is shared by a number of proteins: SH1 being the catalytic domain, SH2 being a phospho-tyrosine binding domain, and SH3 being a proline-binding domain. The Src SH2 domain binds to phospho-Tyr527 (phosphorylated by Csk) within the Src molecule, causing Src to adopt an inactive conformation. Mutation of Tyr527 to phenylalanine removes the SH2-binding target and results in a constitutively active kinase. A second mutation at the conserved lysine residue at the ATP-binding site abolishes kinase activity, but without Tyr527, the conformation of the protein is open, allowing interaction with endogenous activators. Consequently, this double-mutant exhibits a dominant-negative phenotype

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SRC
• Uniprot:
  • P12931()