Src Antibody (4F1E8)

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Western Blot: Src Antibody (4F1E8) [NBP1-47547] - Analysis using SRC mouse mAb against truncated SRC-His recombinant protein (1) and PMA treated THP-1 cell lysate (2).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
4F1E8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Src Antibody (4F1E8) Summary

Immunogen
Purified recombinant fragment of SRC(aa10-193) expressed in E. Coli.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
SRC
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1: 500 - 1:2000
  • ELISA 1:10000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for Src Antibody (4F1E8)

  • ASV
  • c-Src
  • EC 2.7.10
  • EC 2.7.10.2
  • pp60c-src
  • Rous sarcoma
  • RSVgp4
  • Src
  • tyrosine kinase pp60c-src
  • tyrosine-protein kinase SRC-1
  • v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog
  • v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)

Background

Src is a protein tyrosine kinase known to regulate cellular adhesion. Composed of three major domains; SH2, SH3 and a kinase catalytic domain, Src can be switched from an inactive to an active state though control of its phosphorylation state or through protein-protein interaction. Phosphorylation of Y529 by Csk and Chk inactivates Src, while dephosphorylation of Y529 will modify its conformation to an open active state (1-3). Mutation of Y529 leads to Scr overactivity. Several cancers including colon and breast cancer have been associated with an increase of Src activity (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics Tool for Src Antibody (NBP1-47547)

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Androgen Receptor: What Makes a Man?
Steroid receptors (SRs) are a superfamily of ligand-dependent nuclear transcription factors that activate responsive genes response to hormone. Androgen receptors (ARs) are found in a wide variety of tissues, including reproductive organs, central ner...  Read full blog post.

The Heat is On: Heat Shock Proteins and the Link to Cancer
Novus Biologicals offers an extensive antibody catalog targeting heat shock proteins (HSPs). A large protein group covering a number of families, the HSPs are functionally related by their dramatic upregulation in response to stress. Stress triggers m...  Read full blog post.

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Bioinformatics

Gene Symbol SRC
Entrez