SPRR3 Antibody (4A12) - Azide and BSA Free

Western Blot: SPRR3 Antibody (4A12) [H00006707-M01] - Analysis of SPRR3 expression in transfected 293T cell line by SPRR3 monoclonal antibody (M01), clone 4A12.Lane 1: SPRR3 transfected lysate(18.2 KDa).Lane 2: Non-transfected lysate.

Immunohistochemistry-Paraffin: SPRR3 Antibody (4A12) [H00006707-M01] - Analysis of monoclonal antibody to SPRR3 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.

Sandwich ELISA: SPRR3 Antibody (4A12) [H00006707-M01] - Detection limit for recombinant GST tagged SPRR3 is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: 4A12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

SPRR3 Antibody (4A12) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: SPRR3 (AAH17802, 1 a.a. ~ 161 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSSYQQKQTFTPPPQLQQQQVKQPSQPPPQEIFVPTTKEPCHSKVPQPGNTKIPEPGCTKVPEPGCTKVPEPGCTKVPEPGCTKVPEPGCTKVPEPGYTKVPEPGSIKVPDQGFIKFPEPGAIKVPEQGYTKVPVPGYTKVPEPCPSTVTPGPAQQKTKQK
• Specificity: SPRR3 - small proline-rich protein 3
• Isotype: IgG3 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SPRR3
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot
• Application Notes: Antibody reactivity against recombinant protein for WB. It has been used for IHC-P and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SPRR3 Antibody (4A12) - Azide and BSA Free

• 22 kDa pancornulin
• Cornifin beta
• Esophagin
• small proline-rich protein 3
• SPRC

Background

SPRR3, also known as Small proline-rich protein 3, consists of a 169 amino acid isoform that is 18 kDa, and is involved in the surrounding and protection of keratinocytes. Disease research on the protein is currently being conducted with ichthyosis vulgaris, psoriasis, eosinophilic esophagitis, verrucous carcinoma, periodontitis, esophageal cancer, eczema, tonsillitis, and skin disease. SPRR3 is linked to several biological processes, including epidermis development, wound healing, and keratinocyte differentiation, in which it interacts with other proteins, such as IVL, EVPL, NFKBIA, and LOR.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SPRR3 Antibody (H00006707-M01)(2)

Publications using H00006707-M01

• Rita R, Katsumitsu S, Satoshi M et al. Contribution of transglutaminases and their substrate proteins to the formation of cornified cell envelope in oral mucosal epithelium. Eur J Oral Sci. 2021-01-27 [PMID: 33501693]
• Igoh A, Doi Y, Sakurada K. Identification and evaluation of potential forensic marker proteins in vaginal fluid by liquid chromatography/mass spectrometry. Anal Bioanal Chem. 2015-07-12 [PMID: 26164306]

Bioinformatics

• Gene Symbol: SPRR3
• Entrez:
  • 6707(Human)
• OMIM:
  • 182271(Human)
• Uniprot:
  • AAH17802(Human)