SPANXC Antibody

Images

 
Western Blot: SPANXC Antibody [NBP1-03285] - Affinity purified anti-SPANX (pan) antibody shows detection of a band at ~17 kD corresponding to SPANX-C present in a nuclear extract from VWM105 cells (left panel, ...read more
Immunohistochemistry-Paraffin: SPANXC Antibody [NBP1-03285] - Staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ...read more

Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

SPANXC Antibody Summary

Background
Affinity purified anti-SPANX (pan) antibody shows detection of a band at ~17 kD corresponding to SPANX-C present in a nuclear extract from VWM105 cells (left panel, arrowhead). VWM105 cells are derived from a human melanoma and are positive for SPANX proteins. Lane 2 shows reactivity with a purified recombinant SPANX-C fusion protein. The right panel shows similar reactivity with purified re...combinant SPANX-B, SPANX-C and SPANX-N proteins. Proteins were separated by SDS-PAGE, transferred to nitrocellulose, and probed with the primary antibody diluted to 1:1000. IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX ( was used (left). IRDye is a trademark of LI-COR, Inc. Size estimation was made by comparison to prestained MW markers as indicated. Personal Communication. Vladimir Larionov, NIH, CCR, Bethesda, MD.
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Immunogen
Synthetic peptide from human SPANXC.
Specificity
Amino acids 31-47 of human SPANX-C protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SPANXC
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • ELISA 1:10000-1:50000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 5 ug/ml

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Potassium Phosphate (pH 7.2) and 0.15M NaCl
Preservative
0.01% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SPANXC Antibody

  • B1
  • C
  • cancer/testis antigen 11.3
  • cancer/testis antigen family 11, member 3
  • cancer/testis-associated protein CTp11
  • cancer/testis-associated protein of 11 kD
  • cancer-testis-associated protein CTp11
  • CT11.1
  • CT11.2
  • CT11.3
  • CT11.4
  • CTp11
  • dJ171K16.1
  • NAP-X
  • nuclear-associated protein SPAN-Xc
  • SPANX family, member C
  • SPANX
  • SPANXA
  • SPAN-Xa
  • SPANX-A
  • SPANXB
  • SPAN-Xb
  • SPANX-B
  • SPAN-Xc protein
  • SPANX-C
  • SPANX-D
  • sperm protein associated with the nucleus on the X chromosome C
  • sperm protein associated with the nucleus, X chromosome, family member C

Background

Human Sperm Proteins Associated with the Nucleus on X-chromosome (SPANX) are relatively low molecular weight cytoplasmic proteins found in testis and sperm. Their expression in other tissues indicates malignancies. Family members are observed as proteins that range from 15 to 20 kDa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SPANXC
Uniprot