SPAG16 Antibody

Immunohistochemistry-Paraffin: SPAG16 Antibody [NBP1-47220] - Staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Bv, Ca
• Applications: ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

SPAG16 Antibody Summary

• Description: Novus Biologicals Goat SPAG16 Antibody (NBP1-47220) is a polyclonal antibody validated for use in IHC and ELISA. Anti-SPAG16 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-KLHYASYEPTIR corresponding to internal region according to NP_078808.3.
• Epitope: Internal region
• Specificity: This antibody is expected to recognize isoform 1 (NP_078808.3) only.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SPAG16
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:32000
• Application Notes: WB: Preliminary experiments gave bands at approx. 55 kDa band and 22 kDa band in human testis lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 70.8 kDa band according to NP_078808.3. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SPAG16 Antibody

• DKFZp666P1710
• FLJ22724
• FLJ37717
• Pf20 protein homolog
• PF20MGC87036
• sperm associated antigen 16
• sperm-associated antigen 16 protein
• sperm-associated WD repeat protein
• WD repeat domain 29
• WDR29

Background

Cilia and flagella are comprised of a microtubular backbone, the axoneme, which is organized by the basal body and surrounded by plasma membrane. SPAG16 encodes 2 major proteins that associate with the axoneme of sperm tail and the nucleus of postmeiotic germ cells, respectively (Zhang et al., 2007 [PubMed 17699735]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SPAG16 Antibody (NBP1-47220)(1)

Publication using NBP1-47220

• Zhang Z, Zariwala MA, Mahadevan MM et al. A heterozygous mutation disrupting the SPAG16 gene results in biochemical instability of central apparatus components of the human sperm axoneme. Biol Reprod 2007-11-01 [PMID: 17699735]

Bioinformatics

• Gene Symbol: SPAG16
• Entrez:
  • 79582(Human)
  • 66722(Mouse)
• Uniprot:
  • NP_078808.3(Human)