SOX6 Antibody

Immunocytochemistry/Immunofluorescence: SOX6 Antibody [NB110-59987] - Labeling for SOX6 in the notochord of 12 d.p.c. mouse embryos. Detection was done using anti-rabbit-Cy3 conjugated secondary antibodies (red fluorescence). Cell nuceli were counterstained with DAPI (blue fluorescence).

Product Discontinued

SOX6 Antibody Summary

• Immunogen: Reacts with the residues 780-796 [YDDYEDDPKSDYSSENE] of human and mouse SOX6. Sequence 100% conserved between human, mouse, rat and chimpanzee.
• Localization: Nuclear
• Specificity: SOX6
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SOX6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-1:300
  • Western Blot 1:1000-2:000
• Application Notes: Dilutions listed as a recommendation. Optimal dilution should be determined by investigator.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS and 5% Trehalose
• Preservative: No Preservative
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SOX6 Antibody

• HSSOX6
• SOX6
• SOXD
• SRY (sex determining region Y)-box 6
• SRY-box containing gene 6
• transcription factor SOX-6

Background

The SOX (SRY-box containing gene) gene family encodes a group of transcription factors defined by the conserved high motility group (HMG) DNA-binding domain. SOX6 is a transcriptional factor that is specifically expressed in the developing central nervous system and in the early stages of chondrogenesis in mouse embryos. Results indicate that the developmentally regulated transcription factor SOX6 is aberrantly expressed in glioma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SOX6
• Entrez:
  • 20679(Mouse)
  • 293165(Rat)