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SOX13 Antibody

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Western Blot: SOX13 Antibody [H00009580-B01P] - Analysis of SOX13 expression in transfected 293T cell line by SOX13 polyclonal antibody. Lane 1: SOX13 transfected lysate(68.42 KDa). Lane 2: Non-transfected lysate.
Western Blot: SOX13 Antibody [H00009580-B01P] - Analysis of SOX13 expression in transfected 293T cell line by SOX13 polyclonal antibody. Lane 1: SOX13 transfected lysate(68.42 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: SOX13 Antibody [H00009580-B01P] - Analysis of purified antibody to SOX13 on HeLa cell. (antibody concentration 10 ug/ml)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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SOX13 Antibody Summary

Immunogen
SOX13 (NP_005677.2, 1 a.a. ~ 622 a.a) full-length human protein.
Isotype
IgG
Clonality
Polyclonal
Host
Mouse
Gene
SOX13
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunocytochemistry/Immunofluorescence
Application Notes
This antibody is useful for Western Blot, Functional, Immunofluorescence

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
Protein G purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SOX13 Antibody

  • Islet cell antigen 12
  • MGC117216
  • Sox-13
  • SRY (sex determining region Y)-box 13ICA12islet cell 12
  • SRY-box 13
  • SRY-related HMG-box gene 13
  • transcription factor SOX-13
  • Type 1 diabetes autoantigen ICA12

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western blot shows lysates of human brain (hypothalamus) tissue and human brain (hippocampus) tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human IA-2/PTPRN Monoclonal Antibody (Catalog # MAB7906) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IA-2/PTPRN at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of human brain (hypothalamus) tissue, loaded at 0.5 mg/mL. A specific band was detected for IA-2/PTPRN at approximately 78 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human IA-2/PTPRN Monoclonal Antibody (Catalog # MAB7906). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.       
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Western Blot: GAD2/GAD65 Antibody [NBP1-33284] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD65 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301 was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: GAD2/GAD65 Antibody [NBP1-33284] - DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GAD65 stained by GAD65 antibody diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody (NBP2-43559) [11710] diluted at 1:500. Blue: Fluoroshield with DAPI.
Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody [NBP1-33284] - Paraffin-embedded RT2 xenograft; antibody dilution 1:500.
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Species: Hu, Mu, Rt
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Western Blot: HNF1 Antibody [NBP1-33596] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1 alpha antibody [N1N3] diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: HNF1 Antibody [NBP1-33596] - Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HNF1 alpha protein stained by HNF1 alpha antibody diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody (diluted at 1:1000). Blue: Hoechst 33342 staining.
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Immunocytochemistry/Immunofluorescence: Glutamine Synthetase Antibody [NB110-41404] - Immunofluorescence using NB110-41404. Submitted via verified customer review.
Immunohistochemistry: Glutamine Synthetase Antibody [NB110-41404] - Localization of glutamine synthase in the retina. Paraffin sections of mouse (A, B), rat (C, D, G-I), or human (E, F) retina fixed in 4% paraformaldehyde were reacted with anti-glutamine synthase (red fluorescence staining in B, D, G, I, and brown immuno-peroxidase reaction. Nuclei in IF experiments (A-D) were stained with DAPI (cyan), and with nuclear fast red in E and F. At low magnification, anti-glutamine synthase reacted with a single population of cells extending from the ganglion cell layer through the inner nuclear layer. No signal was detected in controls either pre-incubated with 100ug/ml of the immunizing peptide (A) or with pre-immune serum (C, E). This finding was confirmed by co-localization (indicated by yellow in I) of glutamine synthase (red in G) with antoher marker of glutamine synthase (green in H).
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(1 Review)
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SOX13 Antibody
H00009580-B01P
SOX13 Antibody
Species: Hu
Applications: WB, ELISA, ICC/IF

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Bioinformatics

Gene Symbol SOX13
Uniprot